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Preparation method of lycium barbarum glycopeptide with effect of repairing and preventing liver injury induced by anti-tuberculosis drugs

A technology of wolfberry and glycopeptide, applied in the fields of food, health food and medicine, can solve problems such as not being effectively solved, and achieve the effects of improved cell survival rate, good repair and prevention effects, and improved purity

Active Publication Date: 2019-08-30
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many hepatoprotective drugs on the market including silymarin, bicyclol, glycyrrhizic acid-related preparations and other reports for anti-tuberculosis drug-induced liver injury, due to various reasons, this problem has not been effectively resolved so far

Method used

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  • Preparation method of lycium barbarum glycopeptide with effect of repairing and preventing liver injury induced by anti-tuberculosis drugs
  • Preparation method of lycium barbarum glycopeptide with effect of repairing and preventing liver injury induced by anti-tuberculosis drugs
  • Preparation method of lycium barbarum glycopeptide with effect of repairing and preventing liver injury induced by anti-tuberculosis drugs

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Experimental program
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Effect test

Embodiment 1

[0037] (1) Wash and dry the wolfberry fruit, put it in a pulverizer and pulverize it, pass through a 40-60 mesh sieve, flush it into nitrogen and store it in cold storage;

[0038] (2) Take 5kg of the crushed and sieved Lycium barbarum raw materials mentioned above and place them in the extraction bag, put them into the closed extraction tank of the subcritical ultrasonic extraction equipment, close the feeding channel, evacuate to -0.075MPa, open the butane storage tank, and follow the The ratio of solid to liquid is 1:1.5kg / L, feed 0.75L of butane, heat to 35°C, set the extraction pressure to 0.5MPa, the extraction time is 15min, the ultrasonic frequency is 15KHz, and extract 3 times; after the extraction, extract the extraction solvent to in the separation tank. Turn on the vacuum pump, reduce the pressure of the separation tank to -0.06MPa, keep the temperature at 35°C, evaporate the butane in the material, and compress the vaporized butane into a liquid state through a di...

Embodiment 2

[0045] (1) Wash and dry the wolfberry fruit, put it in a pulverizer and pulverize it, pass through a 40-60 mesh sieve, flush it into nitrogen and store it in cold storage;

[0046] (2) Take 5kg of the crushed and sieved Lycium barbarum raw materials mentioned above and place them in the extraction bag, put them into the closed extraction tank of the subcritical ultrasonic extraction equipment, close the feeding channel, evacuate to -0.075MPa, open the butane storage tank, and follow the The ratio of solid to liquid is 1:1.5kg / L, feed 0.75L of butane, heat to 35°C, set the extraction pressure to 0.5MPa, the extraction time is 15min, the ultrasonic frequency is 15KHz, and extract 3 times; after the extraction, extract the extraction solvent to in the separation tank. Turn on the vacuum pump, reduce the pressure of the separation tank to -0.06MPa, keep the temperature at 35°C, evaporate the butane in the material, and compress the vaporized butane into a liquid state through a di...

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Abstract

The invention discloses a preparation method of lycium barbarum glycopeptide with effect of repairing and preventing liver injury induced by anti-tuberculosis drugs. The method subjects lycium barbarum fruits to crushing, sieving, subcritical degreasing, high-speed shearing and low-temperature wall-breaking water extraction, ethanol precipitation, hot water redissolution, high-speed centrifugation, microfiltration membrane purification, nanofiltration membrane concentration, trichloroacetic acid deproteinization, active carbon decolorization and freeze drying so as to obtain the lycium barbarum glycopeptide with effect of repairing and preventing liver injury induced by anti-tuberculosis drugs. The main component analysis shows that the molecular weight of the lycium barbarum glycopeptideis 10kD-5kD, the polysaccharide content is 85% or above, and the uronic acid content is 10% or above. Activity experiments show that the lycium barbarum glycopeptide can enhance the activity of livercells, obviously regulate down the activity of glutamic-pyruvic transaminase and the level of glutamic-oxaloacetic transaminase caused by isoniazid and rifampicin, has better prevention and repair effects on liver injury caused by anti-tuberculosis drugs of rifampicin and isoniazid, and can be used as an effective component for preparing health-care foods and pharmaceutical preparations.

Description

technical field [0001] The invention relates to a preparation method of wolfberry glycopeptide, in particular to a preparation method of wolfberry glycopeptide capable of repairing and preventing liver damage induced by anti-tuberculosis drugs, and belongs to the technical fields of food, health food and medicine. Background technique [0002] On October 30, 2017, in the "2017 Global Tuberculosis Report" released by WHO, it was pointed out that it is estimated that there were about 10.4 million new tuberculosis patients in the world in 2016, and the estimated incidence rate was 142 / 100,000. There were 6.7 million cases in men, 3.7 million in women and 1.04 million in children. Tuberculosis remains one of the top 10 causes of death globally and the number one infectious disease killer. In 2016, the global estimated tuberculosis death was about 1.67 million, and China is one of the 30 countries with high burden of tuberculosis in the world. WHO estimated that there were about...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K1/14C07K1/34A61P1/16
CPCC07K2/00A61P1/16
Inventor 邸多隆刘建飞
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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