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A combination marker and its application in the preparation of gastric cancer risk prediction kit and its measurement system and method

A technology for detecting kits and markers, which is applied in biochemical equipment and methods, microbe determination/testing, biological testing, etc., and can solve problems such as the lack of combined markers

Active Publication Date: 2021-03-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are different pathological stages in the occurrence of gastritis to gastric cancer (atrophic gastritis, intestinal metaplasia, and dysplasia)[11], but there is still a lack of combined markers for the occurrence of gastritis to gastric cancer

Method used

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  • A combination marker and its application in the preparation of gastric cancer risk prediction kit and its measurement system and method
  • A combination marker and its application in the preparation of gastric cancer risk prediction kit and its measurement system and method
  • A combination marker and its application in the preparation of gastric cancer risk prediction kit and its measurement system and method

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Experimental program
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Effect test

Embodiment 1

[0051]The inventors used next-generation sequencing technology to obtain LIF, MIF, CCND2, BCL1L2, Expression of FABP1, ACSL5, ACADS, TBC1D1, CDX2 at gene level.

[0052] After analyzing the reference group of 38 cases of atrophic gastritis, the parameters of the formula are as follows:

[0053]

[0054] Based on the above parameters, the relative expression levels of each of the nine genes in 56 patients were calculated, and the risk grouping of the patients was obtained using the risk index calculation formula in the present invention, which was found to be significantly associated with the clinical stages of the patients (p=0.008457). And compared with the clinical stage, the expression levels of molecules such as LIF and FABP1 were more significantly correlated with the expression levels of CDX2 known to be related to intestinal metaplasia (such as figure 1 shown), suggesting that the combined markers in the present invention may be more closely related to the specific ...

Embodiment 2

[0057] Using the immunohistochemical detection kit according to the present invention to obtain seven molecular LIF, CCND2, BCL1L2, FABP1, ACSL5, The expression of ACADS and TBC1D1 at the protein level (MIF and CDX2 are not detected in this example, and can be directly replaced by zero value during calculation). The kit utilizes immunohistochemistry (IHC) to measure expression levels of the combined markers. 10% buffered formalin was used to fix paraffin-embedded surgical samples, and the tissue section was 4 μm / sheet.

[0058] The test kit in this embodiment includes the following components:

[0059] (1) Reagent A: blocking solution, which is 10% goat serum;

[0060] (2) Reagent B: diluted ready-to-use anti-LIF primary antibody;

[0061] (3) Reagent C: diluted ready-to-use anti-CCND2 primary antibody;

[0062] (4) Reagent D: diluted ready-to-use anti-BCL2L1 primary antibody;

[0063] (5) Reagent E: diluted ready-to-use anti-FABP1 primary antibody;

[0064] (6) Reagent ...

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Abstract

The inventors proposed a method for assessing the risk of gastric cancer patients based on combined markers, and quantitatively measured the risk of gastric cancer according to the expression of the combined markers in case samples. The present invention relates to a combination marker composed of nine proteins LIF, MIF, BCL1L2, CCND2, TBC1D1, FABP1, ACSL5, and ACADS, a detection kit for detecting the combination marker, and a gastric cancer risk assessment and stratification system, combined with the above According to the results of marker detection and risk stratification of patients, it is found that high-risk patients have stomach fire (stomach heat) symptoms such as "overeating fat and sweet taste" and "bad breath", "acid reflux", and "heartburn", and / or "red and white gastric mucosa", "exposed submucosal blood vessels" and other gastroscopic symptoms. The implementation results of 56 and 30 cases of chronic gastritis patients show that the present invention can predict the risk of gastric cancer and provide early warning to gastric precancerous lesions and gastric cancer.

Description

technical field [0001] The invention relates to the application of a combined marker in the preparation of a kit for predicting the risk of gastric cancer, and its measurement system and method. Background technique [0002] Gastric cancer is one of the most common tumors worldwide, especially in Asia, especially China [1]. The prognosis of early gastric cancer is significantly better than that of late [2], so the identification of high-risk groups for gastric cancer has important clinical significance. At present, although studies have revealed some high-risk factors for gastric cancer, such as smoking, nitrite intake, Helicobacter pylori infection, chronic gastritis, etc. [3-5], there is still a lack of effective clinical diagnosis and treatment methods, especially the lack of Effective gastric cancer risk markers to guide clinical diagnosis and treatment[6]. [0003] As a complex process, the occurrence of gastric cancer is not determined by a single gene or its product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574G01N33/68
CPCC12Q1/6886C12Q2600/158G01N33/57446G01N33/68
Inventor 李梢谭艾迪
Owner TSINGHUA UNIV
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