Preparation method of attenuated vaccine of mycoplasma synoviae

A technology for Mycoplasma synovialis and an attenuated vaccine is applied in the field of preparation of attenuated vaccines, and can solve problems such as unsatisfactory effect, inability to eliminate MS infection in chicken flocks, poor drug control effect, etc. The effect of market vacancies

Inactive Publication Date: 2019-08-23
NINGXIA UNIVERSITY +1
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Problems solved by technology

[0003] The control of mycoplasma synovialis is mainly through vaccine immunization and drug control. So far, only Australia and other countries have commercialized MS inactivated vaccine and attenuated vaccine application, and the effect is not very satisfactory
However, farms in m...

Method used

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  • Preparation method of attenuated vaccine of mycoplasma synoviae
  • Preparation method of attenuated vaccine of mycoplasma synoviae
  • Preparation method of attenuated vaccine of mycoplasma synoviae

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Experimental program
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Embodiment 1

[0030] Embodiment 1: the screening of bacterial strain:

[0031] 1 Culture medium for the production of Mycoplasma synovialis

[0032] 1.1 Modified Frey liquid medium for Mycoplasma synovial bursa (purchased from Beijing Zhonghai Biotechnology Co., Ltd.);

[0033] 1.2 Modified Frey solid medium for mycoplasma synovial bursa (purchased from Beijing Zhonghai Biotechnology Co., Ltd.);

[0034] 2 Isolation and identification of mycoplasma in chicken synovial bursa

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Abstract

An H95 attenuated mycoplasma synoviae strain is obtained through in-vitro subculture in order to research an attenuated vaccine capable of preventing mycoplasma synoviae. The H95 strain and a white oil adjuvant are mixed in the proportion of 1:1 to prepare the attenuated vaccine, and the immune effect of the strain on chickens is researched by immunizing the chickens with different immune doses; animal infection research is carried out through a pathogenicity wild strain, so that the prevention effect of the attenuated vaccine prepared from the H95 attenuated strain on mycoplasma synoviae is verified. The invention provides a preparation method of the attenuated vaccine capable of preventing mycoplasma synoviae and an effective immunizing dose. A scientific basis is provided for clinicallypreventing mycoplasma synoviae.

Description

technical field [0001] The invention relates to a preparation method of an attenuated vaccine of Mycoplasma gallinarum. Background technique [0002] Mycoplasma gallinarum ( Mycoplasma synoviae , MS) is an important pathogen that mainly causes swelling of joints and inflammation of synovial bursa and tendon sheath in chickens worldwide. In recent years, the disease has been increasing year by year throughout the country, seriously affecting the healthy development of the poultry industry. Chicken synovial bursa mycoplasma disease can occur all year round, but it is easy to occur in winter and early spring, and chickens of various ages can be affected. The susceptibility of young chickens is higher than that of adult chickens, and the resistance increases with the growth of age. Introduced varieties or strains have a higher incidence than local varieties. The resistance of the affected chicken flocks decreases, and in the early stage of the disease, because the clinical sy...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61P31/04C12N1/20C12R1/35
CPCA61K39/0241A61K2039/522A61K2039/552A61P31/04C12N1/20C12N1/205C12R2001/35
Inventor 何生虎郭朋辉郭亚男姜肖军刘凡陈秀红郭磊魏晓明
Owner NINGXIA UNIVERSITY
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