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Tissue culture propagation method for ludisia discolor

A technology of blood leaf orchid group and blood leaf orchid, which is applied in the field of plant tissue culture, can solve the problems that tissue culture cannot directly use blood leaf orchid and hinder the large-scale production of blood leaf orchid, so as to increase the survival rate of germination and facilitate survival , the effect of step refining

Inactive Publication Date: 2019-08-23
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the rapid propagation technology of blood leaf orchid tissue culture is still in its infancy, which hinders the large-scale production of blood leaf orchid to a large extent.
And the existing tissue culture of other orchid varieties can not be directly applied to the culture of the blood leaf orchid, so there is an urgent need for a blood leaf orchid tissue culture propagation method

Method used

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  • Tissue culture propagation method for ludisia discolor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) material selection: select blood leaf orchid without pests and diseases, good growth and strong plants, and obtain capsules by artificial pollination;

[0026] (2) Capsule pretreatment: select mature capsules 25 days after pollination, and use soft bristles to preliminarily clean the selected capsules in a pool to remove surface silt, then clean them with detergent, and rinse them under running water for 30 minutes;

[0027] (3) Capsule disinfection: On the ultra-clean workbench, soak the capsules with 75% alcohol aqueous solution for 1 min, rinse with sterile water once, and then soak them in 0.15% mercuric chloride aqueous solution for disinfection for 15 min. , rinsed 5 times with sterile water, blotted dry the water on the surface of the capsule with sterile filter paper and placed it in a petri dish for later use;

[0028] (4) Seed germination: cut open the sterilized capsules, distribute the seeds evenly on the seed germination medium for cultivation, first ca...

Embodiment 2

[0032] (1) material selection: select blood leaf orchid without pests and diseases, good growth and strong plants, and obtain capsules by artificial pollination;

[0033] (2) Capsule pretreatment: select mature capsules 25 days after pollination, and use soft bristles to preliminarily clean the selected capsules in a pool to remove surface silt, then clean them with detergent, and rinse them under running water for 30 minutes;

[0034] (3) Capsule disinfection: On the ultra-clean workbench, soak the capsules with 75% alcohol aqueous solution for 1 min, rinse with sterile water once, and then soak them in 0.15% mercuric chloride aqueous solution for disinfection for 15 min. , rinsed 5 times with sterile water, blotted dry the water on the surface of the capsule with sterile filter paper and placed it in a petri dish for later use;

[0035] (4) Seed germination: cut open the sterilized capsules, distribute the seeds evenly on the seed germination medium for cultivation, first ca...

Embodiment 3

[0039] (1) material selection: select blood leaf orchid without pests and diseases, good growth and strong plants, and obtain capsules by artificial pollination;

[0040] (2) Capsule pretreatment: select mature capsules 25 days after pollination, and use soft bristles to preliminarily clean the selected capsules in a pool to remove surface silt, then clean them with detergent, and rinse them under running water for 30 minutes;

[0041] (3) Capsule disinfection: On the ultra-clean workbench, soak the capsules with 75% alcohol aqueous solution for 1 min, rinse with sterile water once, and then soak them in 0.15% mercuric chloride aqueous solution for disinfection for 15 min. , rinsed 5 times with sterile water, blotted dry the water on the surface of the capsule with sterile filter paper and placed it in a petri dish for later use;

[0042] (4) Seed germination: cut open the sterilized capsules, distribute the seeds evenly on the seed germination medium for cultivation, first ca...

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Abstract

The invention discloses a tissue culture propagation method for ludisia discolor. According to the method, through capsule disinfection, seed germination, strong seedling rooting and transplanting, alarge number of high-quality ludisia discolor seedlings are obtained. According to the method, the ludisia discolor can be rapidly cultivated and propagated without adding a plant growth regulator, the cultivated ludisia discolor is high in seedling rate, good in seedling quality and strong in adaptability after cultivation, and is safe and environment-friendly as a medicinal plant, the requirement of commercial production is met in a short time through biological technical means, and a technical support is provided for large-scale and industrialized cultivation of the ludisia discolor in enterprises. Meanwhile, the method is good in disinfection effect, low in pollution rate, simple in steps and short in cultivation period, so that the seedling rate and the working efficiency are greatlyimproved, and resources are saved.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and propagation of orchid. [0003] Background technique: [0004] Ludisia discolor, also known as caddisia, is a perennial herb belonging to Orchidaceae. Blood leaf orchid is a high-grade foliage and flower viewing plant, and it is also a rare medicinal plant. It is recorded in Chinese medical books "Chinese Materia Medica" and "National Compilation of Chinese Herbal Medicine". [0005] Due to the good medicinal value of the blood leaf orchid, its seeds are difficult to germinate under natural conditions, and the regeneration of resources is slow. Coupled with long-term unrestrained excavation, the resources are on the verge of exhaustion. In order to better protect the wild resources of the blood leaf orchid, artificial cultivation should be vigorously developed to meet the market demand and ensure the sustainable ut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
CPCA01G31/00A01H4/001A01H4/008
Inventor 张雯郑希龙李伟杰崔馨云
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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