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Screen method and screen device for protein markers for gastric cancer classification, and application of screened protein marker

A technology of protein markers and screening methods, which can be used in measuring devices, material separation, biological testing, etc., and can solve problems such as inability to choose chemotherapy for patients

Active Publication Date: 2019-08-20
北京谷海天目生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, genome-based methods are still unable to give an effective prognostic assessment of patients' chemotherapy and choice of different drugs

Method used

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  • Screen method and screen device for protein markers for gastric cancer classification, and application of screened protein marker
  • Screen method and screen device for protein markers for gastric cancer classification, and application of screened protein marker
  • Screen method and screen device for protein markers for gastric cancer classification, and application of screened protein marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0184] Part 1, Sample preparation method based on paraffin-embedded samples

[0185] Paraffin packs for 50 known gastric cancer cases (specifically including known information such as age, sex, cancer cell ratio, Lauren type, TNM type, survival time, death or not, chemotherapy or chemotherapy regimen, etc.) Buried tissue samples for protein extraction and analysis, the detailed steps are as follows:

[0186] (1), slice:

[0187] Take the embedded wax block, use a scalpel to remove excess paraffin around the tissue block, and trim the tissue block into a trapezoid;

[0188] Fix the wax block on a microtome, adjust the slice thickness to 5 microns, and slice;

[0189] Use a brush or a toothpick to pick the slices and put them in a film spreader at a temperature of 45°C;

[0190] Use glass slides to pick up slices (take at least two slides, one for the experiment and one for spare), after the wax slices are aligned, number them with a pencil, place the slides vertically to dry...

Embodiment 2

[0241] Based on the typing results of Example 1, this example further tested the sensitivity of patients with different types of gastric cancer to chemotherapy, the sensitivity of drug use, and the relationship between survival and prognosis, wherein, Figure 4 The relationship between the survival of patients with C6 classification and the prognostic survival rate of chemotherapy treatment is shown.

Embodiment 3

[0243] Using the typing results established in Example 1, the 224 newly collected gastric cancer samples were tested for typing diagnosis one by one, and the steps of protein separation and mass spectrometry separation were the same as in Example 1. The steps of typing detection are as follows:

[0244] 1. Protein accuracy screening. The proteins detected in the test samples are screened, and the proteins with at least two high-quality peptides detected and one of them is the only high-quality peptide, or the proteins with at least three high-quality peptides detected are retained.

[0245] 2. Protein frequency screening. If the protein detected in the sample to be tested has appeared in 80% of the samples in the known gastric cancer sample set in Example 1, the protein will be retained.

[0246] 3. According to the parameters of standardized protein concentration in known gastric cancer samples in Example 1, standardize the protein after the above-mentioned screening in the...

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Abstract

The invention provides a screen method and screen device for protein markers for gastric cancer classification, and application of a screened protein marker. The screen method comprises steps of screening proteins meeting retaining conditions from a protein expression mass spectrometry database formed by multiple samples, and using the proteins as an effective protein set; performing twice dimension reducing processing on the effective protein set in sequence, so as to obtain a dimension-reduced protein set; performing clustering analysis on the dimension-reduced protein set, so as to obtain different types of protein markers. In the method, protein markers with remarkable high expression in cancer samples are screened out from a protein mass spectrometry database comprising a large quantity of gastric cancer samples, gastric cancer falls into different types of protein markers according to a relationship between different protein markers and survival rates, and the markers of different types have remarkable differences, helping classify gastric cancers more accurately.

Description

technical field [0001] The present invention relates to the field of tumor detection, in particular to a method for screening protein markers for gastric cancer typing, a screening device and an application of the protein markers for screening. Background technique [0002] Gastric cancer ranks third among the causes of cancer death in the world. my country is one of the areas with a high incidence of gastric cancer, and the number of new cases every year is close to half of that in the world. At present, there are mainly three methods for evaluating the prognosis of gastric cancer: the first is the Lauren classification based on histomorphology, which divides gastric cancer into intestinal type, diffuse type, and mixed type. Among them, intestinal type gastric cancer has the best prognosis, with a 5-year survival rate of 65%; diffuse type gastric cancer has the worst prognosis, with a 5-year survival rate of 48%. The second is TNM staging, which stages gastric cancer patie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N33/574G01N33/68
CPCG01N30/88G01N33/57446G01N33/6848G01N2030/8831
Inventor 秦钧汪宜刘明伟夏夏宋雷李恺倪晓天
Owner 北京谷海天目生物医学科技有限公司
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