Method for determining four free fatty acids in human dried blood spots by using high performance liquid chromatography tandem mass spectrometry hyphenated technique

A high-performance liquid chromatography, free fatty acid technology, applied in the field of analysis and detection, can solve the problems of low detection sensitivity and complicated operation

Inactive Publication Date: 2019-08-20
上海可力梅塔生物医药科技有限公司 +3
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  • Claims
  • Application Information

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Problems solved by technology

[0005] However, although there are some detection methods for free fatty acids in the prior art, the operations are often complicated (such as extraction steps are required), the detection sensitivity is low, and it is difficult to quantitatively detect multiple free fatty acids at the same time

Method used

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  • Method for determining four free fatty acids in human dried blood spots by using high performance liquid chromatography tandem mass spectrometry hyphenated technique
  • Method for determining four free fatty acids in human dried blood spots by using high performance liquid chromatography tandem mass spectrometry hyphenated technique
  • Method for determining four free fatty acids in human dried blood spots by using high performance liquid chromatography tandem mass spectrometry hyphenated technique

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Embodiment 1

[0083] 1. Material

[0084] The samples of the methodological research experiment are derived from the dried human blood spots of human whole blood provided by Beijing Boerxi Technology.

[0085] (1) Instrument: Mass Spectrometry: Shimadzu 8045; Liquid Chromatography: Shimadzu LC-20AD; Vortex Vibration: Multi-Tube Vortex Mixer MTV100, Microplate Shaker MX100-4A from Hangzhou Aosheng Instrument Co., Ltd.; Nitrogen Blowing instrument Agela Technologes 96-hole nitrogen blowing instrument; glass equipment, etc.

[0086] (2) Reagent consumables: chromatographic grade methanol; Agilent ZORBAX Eclipse Plus C18 3*100mm; Agilent ZORBAX Eclipse Plus C18 3*100mm 3.5μm.

[0087] (3) Standard product: Decatriene octaacid- 13 C18 was purchased from Medicalisotopes with a purity of ≥95%, and eicosapentaenoic acid-d5 and docosahexaenoic acid-d5 were purchased from Sigma, with a purity of ≥95%.

[0088] 2. Method

[0089] (1) Chromatographic conditions: mobile phase A: 0.1vt% saturated formic acid in wa...

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Abstract

The invention provides a method for determining four free fatty acids (alpha-octadecatrienoic acid, eicosapentaenoic acid, omega3-docosapentaenoic acid and docosahexaenoic acid) in human dried blood spots by using a high performance liquid chromatography tandem mass spectrometry hyphenated technique. The method comprises the steps that four free fatty acids are separated by high performance liquidchromatography; mass spectrometry isotope internal standard quantitation is used; the concentration of the standard is taken as the X-axis, and the peak area ratio of the standard to the internal standard is the Y-axis to establish a calibration curve; and the content of the four free acids in human dried blood spots is calculated. The method provided by the invention has the advantages of high sensitivity, high specificity, accuracy, simple pretreatment process, short time and improved detection efficiency.

Description

Technical field [0001] The present invention belongs to the technical field of analysis and detection. Specifically, the present invention relates to a method for the determination of four free fatty acids in human dried blood spots using high performance liquid chromatography tandem mass spectrometry technology. Background technique [0002] Free fatty acids (FFAs) play an important role in tissue energy supply, especially in the fasting state. Normal free fatty acid levels are very important to human health. Different free fatty acids play different roles in physiological and pathological conditions, such as eicosapentaenoic acid (C20:5) and docosahexaenoic acid (C22). : 6) It has a certain protective effect on inflammation, cancer, arteriosclerosis, insulin resistance and type 2 diabetes. [0003] Epidemiological studies have shown that fatty acids have a certain correlation with chronic diseases such as insulin resistance, cardiovascular disease, type 2 diabetes, hypertension,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 邱碧涵官培龙朱监宝于嘉屏
Owner 上海可力梅塔生物医药科技有限公司
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