Solid phase extraction-gas chromatography-tandem mass spectrometry detection method for isofenphos-methyl in fruit and vegetable

Abstract

The invention discloses a solid phase extraction-gas chromatography-tandem mass spectrometry detection method for isofenphos-methyl in fruit and vegetable. A sample is prepared, the sample to be purified is extracted, a sample solution to be purified is purified, a standard work solution is prepared, the sample solution to be detected is detected, and the content of isofenphos-methyl in the fruitand vegetable sample is calculated. Thus, the method can be used to determine the isofenphos-methyl pesticide in the fruit and vegetable. The fruit and vegetable sample is extracted by acetonitrile, and purified by a GCB / NH2 solid-phase extraction column, a target analyte is analyzed qualitatively in a multi-reaction monitoring mode by comparing retention time and ion pair information, and an external standard method is quantitative. The method is simple in the experimental process, and high in analysis speed resistance to background interference, sensitivity, reproducibility and accuracy, andcan serve as a new detection method for evaluating the quality of the fruit and vegetable sample.

Description

technical field [0001] The invention relates to the field of food detection, and relates to a solid-phase extraction-gas chromatography-tandem mass spectrometry method, in particular to a method for detecting isofenphos-methyl in vegetables and fruits. Background technique [0002] Pesticide refers to a substance or several substances that are used to prevent, eliminate or control diseases, insects, grasses and other harmful substances that endanger agriculture and forestry, as well as to regulate the growth of plants and insects by chemical synthesis or derived from biological and other natural substances. mixtures of substances and their preparations. Except for a small number of organic crops that do not use pesticides, almost all crops are inseparable from pesticides. The classification of pesticides can be divided into insecticides, fungicides, acaricides and plant growth regulators according to their uses. [0003] Isofenphos-methyl is a highly toxic organophosphorus...

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Problems solved by technology

[0004] However, the current detection method can only be detected by gas chromatography, which can only be qualitatively and quantitatively determined by retention time and response peak area, and lacks a substantial basis for judgment; while the gas chromatography-mass spectrometer used in the relevant literature can only be determined by retention time, mass spectrometry Qualitative and quantitative analysis based on graph and selected ion peak area. Although the qualitative determination information is rich, the purification requirements are high, especially for samples with complex matrix background interference, which cannot meet the qualitative and quantitative requirements. Both of the above two detection methods are prone to missed judgments. and miscalculation

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