Method for detecting N-acetylaspartic acid by using nuclear spin single-state selectivity

A technology of aspartic acid and nuclear spin, which is applied in the field of magnetic resonance, can solve the problems of inconvenient and fast, real-time selective detection of N-acetylaspartic acid, etc., and achieve short test time and simple and fast test method , good selectivity and reproducibility

Active Publication Date: 2019-08-20
EAST CHINA NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are not convenient and quick enough, and cannot realize the real-time selective detection of N-acetylaspartic acid in living organisms.

Method used

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  • Method for detecting N-acetylaspartic acid by using nuclear spin single-state selectivity
  • Method for detecting N-acetylaspartic acid by using nuclear spin single-state selectivity
  • Method for detecting N-acetylaspartic acid by using nuclear spin single-state selectivity

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Embodiment 1

[0035] The specific steps from preparation of N-acetylaspartic acid singlet to realization of N-acetylaspartic acid signal filtering (the instrument used is BrukerAVANCE III 500MHz nuclear magnetic resonance instrument):

[0036] Step 1: In this example, N-acetylaspartic acid is dissolved in D 2 O system, prepare an N-acetylaspartic acid deuterium aqueous solution with a mass fraction of 0.6% and apply a 90° pulse to obtain figure 1 N-Acetyl Aspartate 1 H spectrum, where the triplet at 4.63 ppm is the signal of H on the methine of N-acetylaspartate, the single peak at 4.69 ppm is the water peak signal, and the group of peaks at 2.82 ppm is the N-acetyl sky Two H signals on the methylene group of the aspartic acid molecule. Select the three Hs on the methylene and methine groups in the structure of N-acetylaspartic acid (such as figure 2 Shown 1 The peaks of No. 1, 2, and No. 3 peaks marked on the H spectrum are used as the target peaks, calibrated to the middle of No. 2 and No. 3...

Embodiment 2

[0064] The specific steps from preparing N-acetylaspartic acid singlet under the mixed system to realizing N-acetylaspartic acid signal filtering (the instrument used is BrukerAVANCE III 500MHz nuclear magnetic resonance instrument):

[0065] Step 1: Configure a deuterium aqueous solution of N-acetylaspartic acid and glucose with a mass fraction of 0.6%, place it in a nuclear magnetic resonance instrument, and apply a 90° pulse to obtain Figure 7 Mixed solution shown 1 H spectrum, the triplet at 4.63ppm is the signal of H on the methine of N-acetylaspartate, the single peak at 4.69ppm is the water peak signal, and a set of peaks at 2.82ppm is N-acetylaspartate The two H signals on the methylene of the acid molecule, the rest are the glucose signals. Read the J coupling value between the hydrogens of the system and the corresponding chemical shift:

[0066] ω 1 =899.13Hz,ω 2 =0.39Hz,ω 3 =-0.99Hz;

[0067] J 12 = 6.26 Hz, J 13 =6.15Hz,J 23 = 17.22 Hz.

[0068] Step 2: In the mixed sys...

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Abstract

The invention discloses a method for detecting N-acetylaspartic acid by using nuclear spin single-state selectivity. According to the method, the nuclear spin single states of three H atoms prepared on the methylene and methine of the N-acetylaspartic acid are prepared, the observation of selectivity in a complex mixed system for N-acetylaspartic acid signals is realized according to a characteristic that the nuclear spin single states are not influenced by a gradient field. The measurement method has the advantages of high sensitivity, high accuracy, small interference, high selectivity, highreproducibility and the like. The method can inhibit the signals of other related interference substances in a system with complex compositions and finally obtain the signals of the N-acetylasparticacid which are required to be observed; and the method is expected to monitor the content and distribution of the N-acetylaspartic acid in the living body of a living thing and has a high applicationvalues in related production and life fields such as the biology and medicine fields.

Description

Technical field [0001] The present invention relates to the field of magnetic resonance technology, in particular to a method for preparing the nuclear spin singlet of N-acetylaspartic acid and selectively detecting it by a magnetic resonance instrument, which can realize the detection of N-acetylaspartic acid NMR 1 The H signal is selectively detected. Background technique [0002] N-acetylaspartic acid is the most concentrated neuropeptide in the human brain, and its molecular formula is C 6 H 9 NO 5 It is the product of aspartic acid after N-acetylation, and it is also a specific metabolite of the central nervous system. It is maintained at a very high concentration level in the central nervous system of the organism, and the content in the brain can reach 5-10mmol / L. The concentration of N-acetylaspartic acid is thought to reflect the activity of neurons, and the decrease in its level often indicates neuron loss or atrophy of axons and dendrites, decreased synaptic density o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 李毅辛家祥刘慧霞魏达秀王嘉琛姚叶锋
Owner EAST CHINA NORMAL UNIV
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