Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Common camellia oleifera SSR molecular marker primers and marking method and application thereof

A technology of molecular markers and Camellia oleifera, which is applied in biochemical equipment and methods, microbe measurement/testing, DNA/RNA fragments, etc., can solve the problems of rapid cell division

Active Publication Date: 2019-08-20
QINZHOU UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on the transcriptome and SSR markers of common camellia oleifera root. Camellia oleifera root grows fast and cells divide rapidly. The development and transfer of transcriptome sequencing and SSR molecular markers of common camellia oleifera root development will be able to lay a solid foundation for future development. Provide new ideas for genetic breeding, genome mapping, gene mapping and identification of species kinship

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Common camellia oleifera SSR molecular marker primers and marking method and application thereof
  • Common camellia oleifera SSR molecular marker primers and marking method and application thereof
  • Common camellia oleifera SSR molecular marker primers and marking method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1. Experimental method:

[0035] 1. RNA extraction method of sample camellia oleifera:

[0036] 1) Experimental materials for transcriptome sequencing: the experimental Camellia oleifera variety is the common Camellia oleifera variety "Cenruan No. 3".

[0037] 2) Sample processing: three key time nodes in the rooting process of "Cenruan No. 3" in the tissue culture process: before rooting (CK group), 1 day after rooting (T1 group), and 14 days after rooting That is, [appear root point] (T2 group), 50 days after inserting and rooting [root growth stage] (T3 group), after obtaining the root material, use ddH 2 After rinsing the agarose, put it into liquid nitrogen immediately, and wait for the RNA to be extracted.

[0038] 3) Extraction and sequencing method: Use RNAiso for polysaccharide-rich Plant Tissue to extract RNA from all samples, and mix equal amounts of CK, T1, T2 and T3 to construct a cDNA library for Illumina HiSeq2000 sequencing.

[0039] 2. Experimental m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the technical field of molecular marking and particularly relates to common camellia oleifera SSR molecular marker primers and a marking method and an application thereof. The common camellia oleifera roots are subjected to RNA extraction, sequencing and assembly, MISA is used to search SSR sites, corresponding primers are designed, camellia oleifera genome DNA isused as a material, the designed SSR primers are validated, and the corresponding primers are obtained from the designed 6,738 pairs of the primers. The primers show polymorphism, can successfully distinguish the camellia oleifera materials of different varieties, provide the new molecular markers for molecular marking auxiliary breeding of the camellia oleifera, and provide new ideas for future researches on genetic breeding, genome mapping, gene mapping, species genetic relationship identification, etc.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of molecular markers, in particular to a common Camellia oleifera SSR molecular marker primer and a marker method and application. [0003] 【Background technique】 [0004] Ordinary Camellia oleifera is the woody oil plant with the most extensive planting area and the highest total output among Camellia oleifera species, and is often used to extract high-quality edible camellia oil; in addition, the oil-extracting residue can be further processed for use in chemical, textile, and pesticide industries raw materials. Because its unsaturated fatty acid content is as high as more than 90%, camellia oil is a kind of high-quality edible oil, which has been promoted as a healthy high-grade edible oil by the Food and Agriculture Organization of the United Nations. With the improvement of living standards, people's requirements for edible oil also increase. Camellia oil has become in short supply and it is d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 王鹏良杨利平王华宇
Owner QINZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com