Method for obtaining seedlings of Actinidia kolomikta

A technology for seedlings of kiwifruit and seedlings, which is applied in the field of seedlings of kiwifruit, and can solve the problems of low germination rate and difficult propagation of kiwifruit seeds

Active Publication Date: 2019-08-16
MUDANJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to reproduce the seeds of kiwifruit. It usually needs a long period of dormancy. Under natural circumstances, it can germinate and grow in the third year. After traditional methods of breaking dormancy, such as sand rubbing, hormone treatment, low temperature treatment and other methods, the fastest is also needed. It can only germinate in the next year, and the germination rate is low

Method used

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  • Method for obtaining seedlings of Actinidia kolomikta
  • Method for obtaining seedlings of Actinidia kolomikta
  • Method for obtaining seedlings of Actinidia kolomikta

Examples

Experimental program
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Embodiment 1

[0042] Embodiment 1, establishment of the method for fast obtaining Actinidia kiwifruit seedlings

[0043] 1. Materials and methods

[0044] 1. Experimental materials

[0045] The Gouzao kiwifruit fruit was picked in Qili, Hengdaohezi on August 13, 2017 and July 26, 2018. The picked fruit was soaked in clean water, the seeds were washed out, the skin and pulp were filtered out, and it was naturally exposed to the sun. Let dry and set aside.

[0046] 2. Experimental method

[0047] (1) Disinfection of kiwi fruit seeds

[0048] Soak kiwifruit seeds in clean water for 48 hours, sterilize with 30% (V / V) hydrogen peroxide for 20 minutes, wash with sterile water twice, soak in 70% (V / V) ethanol for 40 seconds, wash with sterile water for 3 times and set aside.

[0049] (2) Preparation of induction medium for kiwifruit seedlings

[0050] Prepare the following respectively:

[0051] A: Add agar 7g / L, sucrose 20g / L

[0052]B: Add MS (Qingdao Haibo Biotechnology Co., Ltd., produc...

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Abstract

The invention discloses a method for obtaining seedlings of Actinidia kolomikta. The method for obtaining the seedlings of the Actinidia kolomikta comprises following steps: (1), 1/3 of seeds of the Actinidia kolomikta are cut off in the longitudinal direction, the seeds are put on an inducing medium for culture, and saplings are obtained, wherein the inducing medium is obtained by adding 6-BA andNAA to a basic medium, concentration of 6-BA in the inducing medium is 0.8-1.0 mg/L, and concentration of NAA in the inducing medium is 0.02 mg/L; (2), the saplings are transplanted and cultured, andthe seedlings are obtained. According to the method, the seeds of the current year are utilized, the seedlings of the Actinidia kolomikta are obtained within three weeks, the germination rate can reach 63.33%, and the method for obtaining the seedlings is fast and has high germination rate.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for obtaining kiwi fruit seedlings. Background technique [0002] Dog jujube kiwifruit (Actinidia kolomikta (Maxim.et Rupr.) Maxim.), also known as dog jujube, is a vine of Actinidia genus Actinidia, and its distribution range is narrow. It is one of the most cold-resistant kiwifruit species in East Siberia and the Far East of Japan, Russia, and is a precious genetic resource for cold-resistant breeding. Dog jujube kiwi fruit is sweet and sour, fragrant, soft and juicy. It is rich in a variety of amino acids, minerals and vitamins necessary for the human body, especially the content of vitamin C is very high, and has good nutritional value. Studies have shown that kiwi fruit has many health care functions such as anti-oxidation, anti-aging, and anti-cancer. The leaves of Actinidia kiwifruit change dynamically from white to light pink to red during the growing season...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李然红刘丹陈鑫卢招娣王立凤柴军红安玉婷董世鹏
Owner MUDANJIANG NORMAL UNIV
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