High-flux medicine automatic screening device and method
An automatic screening and high-throughput technology, applied in biochemical equipment and methods, measuring devices, biochemical cleaning devices, etc., can solve the problems of expensive production costs, reduce experimental costs, expand utilization, and improve screening efficiency Effect
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[0047] Taking the drug screening of breast cancer cells as an example, the specific method of using the device of the present invention is explained in detail, figure 1 Schematic diagram of dropping the hydrogel solution with breast cancer cells into the well plate.
[0048] The preparation method of the hydrogel solution with breast cancer cells is as follows: the cells in the logarithmic growth phase were washed with 5 mL of PBS to remove the metabolites in the cell growth phase, and then 3 mL of trypsin with a concentration of 0.25% was added to the React at 37 degrees for three minutes, let the adherent cells be in a free state, add 3mL of medium trypsin to stop the digestion of the cells, put them in a centrifuge tube and centrifuge for a few minutes, let the cells sink in the centrifuge tube Below, take out the centrifuge tube, pour off the supernatant, add the medium mixed with hydrogel, and then use the counting plate to count the number of cells, so that the hydrogel ...
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