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Activated partial thromboplastin time measuring reagent and application thereof

A technology of thromboplastin time and reagent, which is applied in the field of determination of activated partial thromboplastin time and activated partial thromboplastin time of reagents

Inactive Publication Date: 2019-08-09
深圳优迪生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, APTT assay reagents with better properties still need further research and development.

Method used

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  • Activated partial thromboplastin time measuring reagent and application thereof
  • Activated partial thromboplastin time measuring reagent and application thereof
  • Activated partial thromboplastin time measuring reagent and application thereof

Examples

Experimental program
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preparation example Construction

[0032] In addition, the present invention also provides a preparation method for an activated partial thromboplastin time (APTT) assay reagent, comprising the steps of:

[0033] 1) Configure the buffer solution and stir evenly;

[0034] 2) Add the buffer salt into the buffer solution and stir evenly;

[0035] 3) Add the activator to the solution obtained in step 2), and stir evenly;

[0036] 4) Add the protective agent to the solution obtained in step 3), and stir evenly;

[0037] 5) Add phospholipids to the solution obtained in step 4), and stir evenly;

[0038] 6) Add the preservative to the solution obtained in step 5), and stir evenly to obtain an activated partial thromboplastin time (APTT) reagent.

[0039] In a specific embodiment of the present invention, the present invention provides a kit, which includes activated partial thromboplastin time (APTT) reagent and calcium chloride solution. Thus, the kit of the present invention can be used to realize APTT detection...

Embodiment 1

[0056] The preparation of embodiment 1 activated partial thromboplastin time (APTT) reagent

[0057] The present embodiment provides preparation of activated partial thromboplastin time (APTT) reagent according to the following formula, including the following groups:

[0058] Experimental group 1 was prepared according to the following steps to obtain the activated partial thromboplastin time (APTT) reagent

[0059] Step (1): the preparation of PBS damping fluid: take by weighing Na 2 HPO 4 .12H 2 O 6.78g, NaH 2 PO 4 .2H2 O0.16g, add 1000mL purified water, stir to make it fully dissolved, the pH value should be in the range of 8.0-9.5;

[0060] Step (2): Add MgAl to the buffer of step (1) 2 (SiO 3 ) 4 2g, stirred to fully dissolve;

[0061] Step (3): Add 0.03 g of ellagic acid to the solution in step (2), stir to dissolve it fully, and adjust the pH to 7.2-7.8;

[0062] Step (4): Add 10.0 g of bovine serum albumin to the solution in step (3), and stir to fully diss...

Embodiment 2

[0076] Embodiment 2 Activated partial thromboplastin time (APTT) kit assembly

[0077] Example 2 provides several kits, which include: activated partial thromboplastin time (APTT) reagent and calcium chloride reagent.

[0078] Wherein, the activated partial thromboplastin time (APTT) reagents were prepared from experimental groups 1-2 of Example 1, respectively.

[0079] The concentration of calcium chloride in the calcium chloride reagent is 0.02M: Weigh 2.22g calcium chloride, Proclin300 1.0g, add it to 1000mL purified water, stir to make it fully dissolve, and make the final concentration of calcium chloride 0.02M calcium chloride reagent. Then dispense into 2mL / bottle.

[0080] The activated partial thromboplastin time (APTT) reagents prepared by the experimental groups 1, 2 and the comparative experimental group were assembled with 2 mL / bottle of calcium chloride reagent respectively to obtain kit 1, kit 2 and kit 3.

[0081] Then the kit was refrigerated at 2-8°C for ...

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Abstract

The invention relates to an activated partial thromboplastin time measuring reagent and application thereof. The activated partial thromboplastin time measuring reagent is prepared from an activator,phospholipids, a buffering agent and a protective agent, wherein the activator is ellagic acid. The activated partial thromboplastin time measuring reagent not only has good sensitivity to heparin, but also has stable and reliable detection results, can be effectively used for clinical monitoring of heparin anticoagulant therapy, and has broad application prospects.

Description

technical field [0001] The present invention relates to the technical field of in vitro diagnostic reagents. Specifically, the present invention relates to activated partial thromboplastin time assay reagents and applications thereof. More specifically, the present invention relates to activated partial thromboplastin time assay reagents and activated partial thromboplastin time assay reagents. Kit and method for measuring activated partial thromboplastin time. Background technique [0002] The process of coagulation is that blood changes from liquid to gel state, and its essence is that soluble fibrinogen is converted into insoluble fibrin. Activated partial thromboplastin time (APTT) is a screening test to check whether the body's endogenous systemic coagulation function is barrier-free, and it is an activity index reflecting coagulation factors XII, XI, IX, VIII, X, V, II and I. The principle of APTT detection is: fully mix the activated partial thromboplastin time (APTT...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86G01N33/573
CPCG01N33/86G01N33/573G01N2333/90G01N2800/224
Inventor 艾峰刘丹
Owner 深圳优迪生物技术有限公司
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