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Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method

A technology of fucoidan and probiotics, which is applied in the direction of being fixed on/in an organic carrier to achieve the effects of improving survival rate, improving biological efficacy and good stress resistance

Active Publication Date: 2019-08-09
SOUTHWEST UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether fucoidan and its hydrolyzed oligosaccharide-encapsulated probiotics have a protective effect has not been reported.

Method used

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  • Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method
  • Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method
  • Application of fucoidin and hydrolyzed oligosaccharide thereof in preparing probiotic protection agent and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the impact of embedding oligosaccharide 1 on the growth of Bacillus subtilis

[0044] Probiotic treatment: Centrifuge the Bacillus subtilis fermentation broth at 4°C and 4000×g for 10 minutes, wash it twice with PBS, suspend the precipitated cells evenly in the same PBS (pH7.0), and store them at 4°C spare.

[0045] Unembedded group: Spin 2ml of concentrated Bacillus subtilis solution with 10ml of pH=7 NaCl solution for 30min to form a monolayer embedded group (simple mixing).

[0046] Fucoidan embedding preparation: 2mL concentrated Bacillus subtilis liquid (20~70*10 7 CFU) was mixed with 10 mL of fucoidan with a concentration of 20 g / L, and rotated for 30 min to make a monolayer embedding group (simple mixing).

[0047] Oligosaccharide 1 embedding preparation: Mix 2 mL of concentrated Bacillus subtilis solution with 10 mL of 20 g / L oligosaccharide 1, and rotate for 30 minutes to make a monolayer embedding group (simple mixing).

[0048]The non-embedde...

Embodiment 2

[0053] Embodiment 2, the impact of embedding oligosaccharide 2 on the growth of Bacillus subtilis

[0054] Probiotic treatment: Bacillus subtilis fermentation broth was centrifuged at 4000×g for 10 min at 4°C, washed twice with PBS, and the pelleted cells were suspended evenly in the same PBS (pH7.0), and stored at 4°C for later use.

[0055] Unembedded group: Spin 2ml of concentrated Bacillus subtilis solution with 10ml of pH7NaCl solution for 30min to form a monolayer embedded group (simple mixing).

[0056] Preparation of fucoidan embedding: Mix 2ml of concentrated Bacillus subtilis solution with 10mL of 40g / L fucoidan, and rotate for 30min to make a monolayer embedding group (simple mixing).

[0057] Oligosaccharide 2 embedding preparation: Mix 2ml of concentrated Bacillus subtilis solution with 10mL of 40g / L oligosaccharide 1, and rotate for 30min to make a monolayer embedding group (simple mixing).

[0058] Treat the non-embedded group, fucoidan-embedded group and oligo...

Embodiment 3

[0063] Example 3, the influence of monolayer embedded oligosaccharide 1 on the growth of Ruvermyces maximus

[0064] Probiotics treatment: centrifuge the fermentation broth of Ruvermyces maximus at 4000×g for 10 min at 4°C, wash it twice with PBS repeatedly, suspend the pelleted cells evenly in the same PBS (pH 7.0), and store them at 4°C for later use.

[0065] Unembedded group: Rotate 2ml concentrated Ruvermyces marx solution with 10ml pH7NaCl solution for 30min to form a monolayer embedded group (simple mixing).

[0066] Preparation of fucoidan embedding: Mix 2ml of concentrated Ruvermyces maximus with 10mL of 40g / L fucoidan, and rotate for 30min to make a monolayer embedding group (simple mixing).

[0067] Preparation of oligosaccharide 1 embedding: Mix 2ml of concentrated R. marxensis solution with 10mL of 40g / L oligosaccharide 1, and rotate for 30min to make a monolayer embedding group (simple mixing).

[0068] Treat the non-embedded group, fucoidan-embedded group and o...

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Abstract

The invention discloses application of fucoidin and hydrolyzed oligosaccharide thereof in preparing a probiotic protection agent and a method. According to the application and the method, the fucoidinand the hydrolyzed oligosaccharide thereof embed probiotics for the first time, the survival conditions in gastric acid and small intestinal fluid are simulated, it is shown through a result that thesurvival time of the probiotics embedded by the fucoidin and the hydrolyzed oligosaccharide is prolonged, the embedding effect of the hydrolyzed oligosaccharide is better than that of the fucoidin, and a foundation is laid for prolonging the high activity of the probiotics in the gastrointestinal environment.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a method for embedding and protecting probiotics by using fucoidan produced by fermentation and its hydrolyzed oligosaccharide. Background technique [0002] Fucoidan is a polysaccharide containing a rare sugar fucose structure, and its main sources include plant extraction and microbial fermentation. The plant extraction method is mainly extracted from brown algae and some marine invertebrates. It has a unique water-soluble polysaccharide combined with sulfate groups, also known as fucoidan sulfate fucoidan; microbial fermentation method is mainly through some Enterobacter species Preparation by fermentation. Existing studies have shown that fucoidan has two-way regulation of immunity, scavenging free radicals, anti-aging, anti-coagulation and anti-thrombosis, anti-tumor and HIV virus, eliminating gastrointestinal system disorders, anti-allergy, enhancing liver function, and reducing...

Claims

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Application Information

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IPC IPC(8): C12N11/10
CPCC12N11/10
Inventor 邹祥谌颉刘超李姗姗夏海钦王振宇
Owner SOUTHWEST UNIVERSITY
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