Cosmetic composition comprising extract of medicinal herbs as active ingredient
A technology of active ingredients and compositions, which is applied in the field of cosmetic compositions, can solve the problems of harmful tissue cells and non-cellular components, chronic inflammation, tissue damage, etc., and achieve excellent skin elasticity enhancement or wrinkle improvement, high application, and skin The effect of problem improvement
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preparation example 1
[0082] Preparation Example 1: Preparation of Longan Meat, Vitex and / or Agarwood Extract
[0083] Longan meat, Vitex, and agarwood were dried and finely chopped, and 100 g of dry weight was put into a flask and extracted by cold immersion in 1000 g of extraction solvent (distilled water) for 3 days. The cold-soaked extracts were filtered through a filter with a pore size of 0.2 μm to prepare extracts of longan meat, vitex and agarwood extracts, respectively.
[0084] And, the mixed extract was prepared by mixing each of the longan meat extract, Vitex extract, and Agarwood extract prepared in Preparation Example 1. The preparation ratios of the mixed extracts are exemplified in Table 1.
[0085] [Table 1]
[0086] Element Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Longan Meat 3 1.5 1.5 1 Vitex 3 1.5 1.5 1 Agarwood 3 1.5 1.5 1
preparation example 2
[0087] Preparation Example 2: Preparation of Essence Containing Longan Meat, Vitex and / or Agarwood Extracts
[0088] Essences comprising the extracts of Examples 1 to 7 above were prepared. The detailed composition (% by weight) constituting the essence is shown in Table 2 below.
[0089] [Table 2]
[0090] Essence composition Essence of the present invention comparative example Longan, Vitex and / or Agarwood Extracts 3 - Triethanolamine 0.25 0.25 Carboxyvinyl Polymers (1) 0.22 0.22 glycerin 4 4 Propylene Glycol 0.2 0.2 parabens 0.2 0.2 beeswax 0.5 0.5 cetostearyl alcohol 1 1 Glyceryl monostearate 1 1 Sorbitan Monostearate (2) 0.5 0.5 Polyethylene glycol monostearate 8 8 Propylparaben 0.1 0.1 Dimethicone 0.3 0.3 Isocetyl octanoate (3) 3 3 squalane 5 5 spices Appropriate amount Appropriate amount pure water Residual amount Residual amount
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experiment example 1
[0094] Experimental example 1: Effect of promoting synthesis of type I collagen in fibroblasts derived from human body - skin elasticity enhancement or wrinkle improvement effect
[0095] The type 1 collagen synthesis promoting effect was confirmed at the cellular level by adding the seven kinds of extracts obtained in Preparation Example 1 above to a culture solution derived from human fibroblasts. The measurement of synthesized collagen was quantified using a PICP EIA kit (Procollagen Type IC-Peptide Enzyme Immuno Assay KIT). In order to measure the amount of collagen synthesis, the extracts of Examples 1 to 7 were added to the medium (DMEM medium) of fibroblasts at a final concentration of 1000ppm and 10000ppm, respectively, and cultured for 48 hours. The kit uses a spectrophotometer to measure the synthesis degree of type 1 collagen at each concentration at 450nm.
[0096] For comparison of the effects, collagen synthesis was measured in the same way for the culture mediu...
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