Preparation method and application of animal blood active complex peptide

Abstract

The invention provides a preparation method of an animal blood active complex peptide. The method combines a membrane separation technology and a biologically directional enzyme digestion technology,can effectively protect animal serum protein and polypeptide activities, and can be used for preparing high-activity animal blood active peptide. According to the method, steps including chemical or high-temperature deformation, spray-drying and the like in a traditional animal blood polypeptide extraction process are removed, a grade-by grade extraction mode is adopted, in the same technical process, an active peptide A (plasma ultrafiltration peptide) contained in plasma is first extracted with a 5000Da ultrafiltration membrane, a plasma protein peptide B (plasma protease digestion peptide)is extracted by using plasma subjected to ultrafiltration and concentration as a raw material and conducting direct enzyme digestion, ultrafiltration and reverse osmosis concentration, an active polypeptide complex formed by combining the active peptide A and the plasma protein peptide B is rich and comprehensive in types, and the two active polypeptides can achieve a biological synergistic effectsince the two active polypeptides are complementary after being mixed. Compared with a relatively single animal polypeptide extracted by a traditional process, the active complex peptide has the advantage of stronger effects in the fields of medical treatment, cosmetology, health protection and the like.

Description

technical field [0001] The patent of the invention belongs to the field of biomedicine, and specifically relates to a preparation method and application of an animal blood active compound peptide. Background technique [0002] Bioactive peptides are composed of amino acids arranged in different compositions, and are a general term for different peptides ranging from dipeptides to complex linear and circular structures. These peptides can be activated by phosphorylation, glycosylation or acylation to exert physiological effects. Peptides are the basic materials that make up all cells in the human body. In the body, various active peptides are produced and secreted in their respective gland cells, and the amount is very small, but peptides are related to human hormones, nerves, reproduction and cell renewal, Various areas of metabolism, growth and repair. Peptides are the basis of life, and life exists because peptides can be produced in their bodies. Cells that lack peptid...

AI Technical Summary

Problems solved by technology

At home and abroad, a large number of animal blood (deer, camel, cattle, sheep blood) peptide powders have been launched on the market, and are widely used in the field of food or health care products in the form of raw materials to improve the body's immunity, anti-fatigue, anti-aging and other abilities, but At present, the traditional extraction process of animal serum peptides mostly uses serum protein as raw material, and is prepared by heating and curing the protein, crushing, proteolytic digestion, ultrafiltration concentration, spray drying and other processes, which not only makes the active peptides contained in the serum lose their activity, The physiological activity of serum active peptides is greatly reduced, and at the same time, various peptides and protease cleaved peptides in serum also lose the physiological activity of peptides after high temperature curing and spray drying