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Pesticide cypermethrin degrading microbial agent and preparation method thereof

A technology of cypermethrin degradation and microbial inoculum, applied in fungi and other directions, can solve the problems of high prevention and control cost, ecological environment pollution, phytotoxicity and the like

Active Publication Date: 2021-05-14
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in the use of chemical pesticides are irrational use of chemical pesticides, serious pesticide residues in agricultural products, high cost of control, serious pollution to the ecological environment, and large-scale pesticide damage.

Method used

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Examples

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preparation example Construction

[0029] The present invention relates to a kind of preparation method of pesticide cypermethrin degrading microbial bacterial agent, comprises the following steps:

[0030] Step 1: Carry out seed slant culture to Penicillium oxalicum SSCL-5 mycelia or spores, obtain Penicillium oxalicum SSCL-5 spore liquid;

[0031] Step 2: Carry out solid fermentation to Penicillium oxalicum SSCL-5 spore liquid;

[0032] Step 3: The solid fermented product is dried and pulverized to obtain solid fermented spore powder, which is mixed with bran and kaolin to prepare the pesticide cypermethrin-degrading microbial bacterial agent.

[0033] Step one is specifically:

[0034] Prepare solid slant medium A, and sterilize at 115°C for 30 minutes, insert Penicillium oxalicum SSCL-5 mycelia or spores into medium A aseptically, place at a temperature of 28-30°C, and continue to cultivate for 72- 96 hours, until a large number of spores are formed on the slope; scrape the spores of Penicillium oxalicum ...

Embodiment 1

[0046] Step 1: Incline cultivation of seeds

[0047] Prepare solid slant medium A, and perform sterilization treatment (115°C, sterilize for 30 minutes), insert Penicillium oxalicum SSCL-5 hyphae or spores into medium A aseptically, place at 28°C, and continue to cultivate After 96 hours, until a large number of spores are formed on the slope; scrape the SSCL-5 spores into a new sterile container, and adjust the spore concentration to 5×10 with sterile 0.85% NaCl aqueous solution 6 Each / gram of Penicillium oxalicum SSCL-5 spore liquid is ready for use.

[0048] The formula of seed slant medium A is as follows:

[0049] Glucose 1.0 g, peptone 8.0 g, potassium dihydrogen phosphate 1.5 g, magnesium sulfate 0.2 g, agar 1.5 g, add water to 100 ml.

[0050] Step 2: Solid fermentation of strains

[0051] Prepare solid medium B, and after routine sterilization, add 1 ml of Penicillium oxalicum SSCL-5 spore liquid obtained in step 1 to medium B aseptically, mix well, keep the consta...

Embodiment 2

[0056] Step 1: Incline cultivation of seeds

[0057] Prepare solid slant medium A and perform sterilization treatment (115°C, sterilize for 30 minutes), insert Penicillium oxalicum SSCL-5 hyphae or spores into medium A aseptically, place at a temperature of 30°C, and continue to cultivate After 72 hours, until a large number of spores are formed on the slope; scrape the SSCL-5 spores into a new sterile container, and adjust the spore concentration to 5×10 with sterile 0.85% NaCl aqueous solution 6 Each / gram of Penicillium oxalicum SSCL-5 spore liquid is ready for use.

[0058] The formula of seed slant medium A is as follows:

[0059] Glucose 1.5 g, peptone 6.0 g, potassium dihydrogen phosphate 0.5 g, magnesium sulfate 0.5 g, agar 1.8 g, add water to 100 ml.

[0060] Step 2: Solid fermentation of strains

[0061] Prepare solid medium B, and after routine sterilization, add 2ml of Penicillium oxalicum SSCL-5 spore liquid obtained in step 1 to medium B aseptically, mix well, ...

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Abstract

The invention relates to a pesticide cypermethrin degrading microbial bacterial agent and a preparation method thereof. In the process of agricultural production, the excessive use of pesticides leads to high pesticide residues in agricultural products and agricultural soil, which brings certain problems to the safety of agricultural products and the sustainable development of agricultural soil. The present invention ferments and produces Penicillium oxalicum SSCL-5 spores in a special solid fermentation medium, collects the fermented product, carries out low-temperature ventilated drying, sieves and pulverizes at low temperature, adds bran and kaolin, stirs and mixes evenly, and obtains the solid preparation powder that is the target product . The invention relates to an efficient and economical environment-friendly pesticide residue treatment technology. The bacterial agent can degrade more than 90% of residual cypermethrin in the soil, and has the advantages of low treatment cost, simple construction, no secondary pollution, and small impact on the environment. .

Description

technical field [0001] The invention belongs to the technical field of soil bioremediation, and in particular relates to a pesticide cypermethrin-degrading microbial bacterial agent and a preparation method thereof. Background technique [0002] As an important means to ensure a good agricultural harvest, chemical pesticides play an important role in agricultural production. The main problems in the use of chemical pesticides are irrational use of chemical pesticides, serious pesticide residues in agricultural products, high control costs, serious pollution to the ecological environment, and large-scale phytotoxicity. [0003] Microbial remediation is a biological measure that uses the metabolism of specific microorganisms to absorb, transform, remove or degrade chemical pesticide components to achieve environmental purification and ecological effect recovery. It has the characteristics of high efficiency, economy, and no secondary pollution. This technology has been applie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A62D3/02A62D101/04A62D101/22A62D101/26A62D101/28
CPCA62D3/02A62D2101/04A62D2101/22A62D2101/26A62D2101/28
Inventor 陈锐孙晓宇瞿佳沈卫荣赵玲侠门欣
Owner MICROBIOLOGY INST OF SHAANXI
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