Application of ddx20 in the preparation of postoperative prognosis assessment kit for clear cell renal cell carcinoma

A technology of renal clear cell carcinoma and DDX20, which is applied in the field of application of DDX20 in the preparation of postoperative prognosis assessment kits for renal clear cell carcinoma, which can solve the problems of lack of specific molecular targets and unclear molecular mechanisms in the treatment

Active Publication Date: 2022-04-05
厦门柏恒生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0006] Clear cell renal cell carcinoma is one of the most threatening tumors to human health. The molecular mechanism of its occurrence is still unclear, and its treatment lacks specific molecular targets. As a very important tumor oncogene, DDX20 At present, there is no literature report that DDX20 is related to the prognosis of clear cell renal cell carcinoma or the metastasis of clear cell renal cell carcinoma

Method used

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  • Application of ddx20 in the preparation of postoperative prognosis assessment kit for clear cell renal cell carcinoma
  • Application of ddx20 in the preparation of postoperative prognosis assessment kit for clear cell renal cell carcinoma
  • Application of ddx20 in the preparation of postoperative prognosis assessment kit for clear cell renal cell carcinoma

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Experimental program
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Embodiment 1

[0042] (a) Pathological specimens were obtained from biopsies or intraoperative and postoperative pathological materials of patients with clear cell renal cell carcinoma.

[0043] (b) The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0044] (c) Paraffin sections of renal clear cell carcinoma tissues were prepared, and the oven was set at 60° C. overnight.

[0045] (d) Dewaxing of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[0046] (e) Section hydration. Soak in sequence: absolute ethanol: 3min; 90% (v / v) ethanol: 3min; 80% ethanol: 3min; 75% ethanol: 3min.

[0047] (f) Washing with PBS 3 times, 5 min each time.

[0048] (h) EDTA antigen high-pressure repair: soak the slices in 0.01M EDTA repair solution, soak in boiling water for 5 minutes, and cool to room temperature. Wash 3 times with PBS, 5min each time.

[0049] (I) Add 300 μL of 3% (w / w) hydrogen peroxide aqueous solution, 37° C. ...

Embodiment 2

[0065] A postoperative tumor sample of clear cell renal cell carcinoma was taken for paraffin-embedded section, and the immunohistochemical method described above was used for detection. After calculation, the DDX20 immunohistochemical score of the cancer tissue was 0.632. After postoperative follow-up, it was found that the patient had recurrence and metastasis of renal clear cell carcinoma at the 40th month after the operation, and died 48 months after the operation.

Embodiment 3

[0067] A postoperative tumor sample of clear cell renal cell carcinoma was taken for paraffin-embedded section, and the above-mentioned immunohistochemical method was used for detection. After calculation, the DDX20 immunohistochemical score of the cancer tissue was 0.299. After postoperative follow-up, it was found that the patient was alive without metastasis and recurrence 5 years after the operation.

[0068] From the above test results, it can be known that detecting the relative expression of DDX20 molecules by immunohistochemistry can predict the risk of distant metastasis of renal clear cell carcinoma and the postoperative survival or death of patients. When the DDX20 immunohistochemical score of cancer tissue is higher than 0.452, clear cell renal cell carcinoma is prone to recurrence and metastasis, and patients with clear cell renal cell carcinoma are prone to die after operation. Obviously, DDX20 is related to clear cell renal cell carcinoma. Therefore, detection o...

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Abstract

The invention provides a new application of DDX20 in the preparation of a postoperative prognosis assessment kit for renal clear cell carcinoma. After extensive and in-depth research, the inventors found for the first time that detecting the relative expression of DDX20 in clear cell renal cell carcinoma tissue by immunohistochemical method can determine the risk of recurrence and metastasis of clear cell renal cell carcinoma in patients with clear cell renal cell carcinoma. The beneficial effects of the present invention are mainly reflected in: the present invention provides the application of DDX20 in the preparation of the postoperative prognosis assessment kit for clear cell renal cell carcinoma, suggesting that the protein can be used to prepare protein molecular markers for judging the prognosis of patients with clear cell renal cell carcinoma. Postoperative monitoring and sequential treatment of patients with clear cell renal cell carcinoma also have important guiding significance.

Description

technical field [0001] The invention relates to the application of DDX20 in the preparation of a postoperative prognosis evaluation kit for renal clear cell carcinoma. Background technique [0002] Renal parenchymal carcinoma is an adenocarcinoma derived from renal tubular epithelial cells, 85% of which are clear cell carcinomas, and some are granulosa cell carcinomas and mixed cell carcinomas. Hemorrhage, necrosis, cystic changes, and calcifications are common in carcinomas. Born in the renal parenchyma, it infiltrates, compresses, and destroys the renal pelvis and calices when it grows up, and develops outside the renal capsule, forming vascular tumor thrombus or metastasizing to lymph nodes and other organs. [0003] The occurrence, development and prognosis of clear cell renal cell carcinoma are closely related to the activation of oncogenes and the inactivation of tumor suppressor genes. It is a complex pathological process induced by multiple factors and involving mul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6893G01N33/6854G01N33/57438G01N33/57484G01N2800/52G01N2800/54G01N2800/50
Inventor 林尧王清水
Owner 厦门柏恒生物科技有限公司
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