Paenibacillus polymyxa, application of paenibacillus polymyxa in preparing dye lignin and method for preparing dye lignin

A polymyx spore-like and genistein technology, which is applied in the research and development of new traditional Chinese medicines, can solve problems such as undiscovered transformation research, and achieve the effects of strong transformation specificity, abundant resources and high transformation efficiency.

Active Publication Date: 2019-07-12
南京师范大学泰州学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the transformation of Paenibacillus polymyxa to genistin in Sophora japonicus

Method used

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  • Paenibacillus polymyxa, application of paenibacillus polymyxa in preparing dye lignin and method for preparing dye lignin
  • Paenibacillus polymyxa, application of paenibacillus polymyxa in preparing dye lignin and method for preparing dye lignin
  • Paenibacillus polymyxa, application of paenibacillus polymyxa in preparing dye lignin and method for preparing dye lignin

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preparation example Construction

[0061] The preparation method of the Sophora japonicus extract in the present invention: take the solid Sophora japonicus powder, fully extract with water according to the solid-liquid ratio, and then filter to obtain the extract. Taking 10% W / V Sophora japonica extract as an example, its preparation method is as follows: take 10 g of solid Sophora japonica powder, fully boil and extract it with 100 ml of water, filter and take the filtrate to dilute to 100 ml.

[0062] The preparation method of the Paenibacillus polymyxa bacteria liquid in the present invention: transfer the strain stored in the refrigerator to a PDA slope for activation, cultivate at 37°C for 36 hours, transfer to the fermentation medium, and shake and cultivate for 2 days at 37°C and 220r / min on a shaking table That is, the bacterial liquid is obtained.

[0063] The preparation method of Paenibacillus polymyxa bacterial powder in the present invention: transfer the bacterial strains stored in the refrigerat...

Embodiment 1

[0073] Prepare 10% W / V, pH5 Sophora japonica extract, inoculate the Paenibacillus polymyxa bacterial solution of the present invention into the above-mentioned transformation substrate according to the inoculum amount of 5% (V / V) for transformation.

[0074] Transformation method: Shake flask culture at 37°C for 4 days.

[0075] After the conversion, centrifuge at 8000rpm, take the supernatant, and obtain the solid product of genistein by conventional extraction methods.

[0076] The content of genistin and genistein before and after conversion was detected by HPLC method. The result is as image 3 with Figure 4 shown.

[0077] figure 2 It is the standard HPLC figure of genistin and genistein. Wherein A is genistin, the retention time is 10.797min, B is genistein, the retention time is 19.01min.

[0078] image 3 It is the HPLC chart of the conversion stock solution of the present invention.

[0079] Figure 4 It is the HPLC figure after conversion of the present in...

Embodiment 2-19

[0086] The biotransformation process of Examples 2-19 is basically the same as Example 1.

[0087] The addition form, dosage, transformation substrate and reaction conditions of the transformation method of the microorganisms used are slightly different, as shown in the table below:

[0088]

[0089]

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Abstract

The invention provides paenibacillus polymyxa. The preservation number of the paenibacillus polymyxa is CCTCC No.M2018877. The invention further provides application of the paenibacillus polymyxain inpreparing dye lignin and a method for preparing the dye lignin through the paenibacillus polymyxain. The paenibacillus polymyxa, the application of the paenibacillus polymyxa in preparing the dye lignin and the method for preparing the dye lignin have the technical effects that the paenibacillus polymyxa capable of converting dye genistin into the dye lignin is discovered for the first time, andcompared with similar paenibacillus polymyxa, the paenibacillus polymyxa has new performance; the conversion specificity is high, and the conversion efficiency is high and can reach 100%; in the method, adopted raw material sophora fruit resources are rich, cheap and easy to obtain, and are ideal raw materials for preparing the dye lignin.

Description

technical field [0001] The invention relates to Paenibacillus polymyxa and its application in preparing genistein and a method for preparing genistein, belonging to the field of research and development of new traditional Chinese medicines. Background technique [0002] Molecular formula of genistein: C 15 h 10 o 5 [0003] Molecular weight: 270.23 [0004] Molecular Structure: [0005] [0006] Genistein is an estrogen-like compound drug, known as phytoestrogens, and is mainly used clinically to treat gynecological diseases and senile osteoporosis (Chinese patent CN101497594A). In addition, in anti-tumor (SanjeevBanerjee et al, 2005, Molecular evidence for increased antitum oractivity of gemcitabine by genistein invitro and invivo using an or the topic model of Pancreatic cancer [J]. Cancer Res. 2005, 65:9064-9072), anti-inflammatory, Labor pain, lowering uric acid (Huang Jingqun et al. Experimental study on anti-inflammatory, analgesic, and lowering uric acid effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/06C12R1/01
CPCC12P17/06C12N1/205C12R2001/01
Inventor 张玉千周学郑丹张宇
Owner 南京师范大学泰州学院
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