Application of novel gastric cancer marker circ-EIF4G3
A marker and molecular marker technology, applied in the field of tumor molecular biology, can solve problems such as poor screening compliance
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[0031] 1. RNase R Digestion and Actinomycin-D Digestion
[0032] 2mg of total RNA was incubated at 37°C for 20 minutes with or without the addition of 3U / mg RNase R, followed by the use of the RNeasyMinElute kit. For actinomycin D treatment, 2 mg of total RNA was incubated at 37°C with or without 1 mg of actinomycin (actinomycin D, Sigma, Chengdu Best Reagent Company), and incubated at 0,6 , 12, and 18 hours to detect the resulting RNA.
[0033] 2. Patient and case screening
[0034]A total of 64 GC tissues and corresponding adjacent non-tumor tissue samples were collected from GC patients, and all tissue samples were obtained from the Department of General Surgery, Nanjing Hospital Affiliated to Nanjing Medical University (between January 2011 and December 2017). All enrolled patients received radiotherapy and chemotherapy before surgery, and their tissue samples were immediately stored in RNA fixation reagent and immediately placed in a -80°C freezer for preservation. Pai...
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