Special multi-channel circular liquid drop matrix preparation device for test tube baby microscope operation dish

Abstract

The invention discloses a special multi-channel circular liquid drop matrix preparation device for a test tube baby microscope operation dish. The device comprises a pressure transmission plate, a filter element and a pipette tip, wherein the pressure transmission plate is of a flat rectangular structure. The device has the advantages that the matrix preparation device is connected with a single-channel pipettor so that daily complex and dull round liquid drop preparation work in each IVF laboratory can become more convenient and quick easily, and operation personnel can be liberated from therepeated mechanical operation and concentrate on more important events; the matrix preparation device is used for synchronously preparing a group of round liquid drops in the same operation dish or culture dish at a time, and the water electrolyte osmotic pressure in the same group of round liquid drops in the same operating dish or the same culture dish is kept highly consistent, so that the accuracy and repeatability of an IVF experiment are ensured, and the device has great significance in efficiently guaranteeing IVF experiment results.

Description

technical field [0001] The invention relates to the technical fields of medicine and biology, in particular to a special multi-channel circular droplet matrix preparation device for a test-tube baby micromanipulation dish. Background technique [0002] The round droplet preparation operation in the operation dish or petri dish in test tube baby technology is one of the most basic, routine and labor-intensive operation steps. The current operation is mainly through a 200ul single-channel manual pipette ( pipette gun) supporting pipetting plastic pipette tips, for pipetting various types of related liquids, preparing various operations, and cultivating droplets, among which round droplets are used for operations or cultivation, because the amount used is relatively large, usually in each conventional 8-10 petri dishes need to be prepared in the operation or petri dish, and in a laboratory with a relatively large workload, the number of such operating dishes or petri dishes tha...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a special multi-channel circular droplet matrix preparation device for IVF (in vitro reproduction) scientific research and clinical production to provide a special multi-channel circular droplet matrix preparation device carrying 8-10 matrix arrays. The problem of a large number of operation dishes or petri dishes with shaped droplets. Under the existing conditions, the operator must use a single-channel manual pipette with a single pipette tip to carefully handle each operation dish or petri dish one by one. Place two rows of 4 or 5 round droplets with a volume range of about 20uL, and then cooperate with other operations to prepare corresponding operation dishes or petri dishes. This method is time-consuming and labor-intensive. It is a serious test of physical strength and endurance, and these round droplets are prepared sequentially, resulting in inconsistencies in the internal water and electrolyte osmotic pressures between the prepared droplets, which also caused downstream IVF experiments. Certain troubles have affected the accuracy and repeatability of the overall IVF experiment. We have designed a special multi-channel circular droplet matrix preparer for IVF to cooperate with the conventional single-channel manual pipetting of various volumes in the IVF laboratory. The device can simultaneously prepare 8-10 circular operation or culture droplets that meet the requirements in the IVF laboratory at one time, which greatly facilitates the preparation of IVF operation dishes or petri dishes conveniently and quickly under high-throughput requirements, ensuring the same operation The high consistency of water electrolyte osmotic pressure inside a group of circular droplets in a dish or petri dish ensures the strict accuracy and high repeatability of various IVF experimental results, and efficiently completes IVF under the requirements of large throughput In the experiment, the preparation of circular droplet matrix operation dish or high-throughput preparation of petri dish can be matched with a variety of automatic pipetting operating systems

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