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Method for extracting flavanomarein and marein in senecio cineraria

An extraction method, the technology of snow chrysanthemum, which is applied in the field of aflatoxin and maritin, can solve the problems of difficult extraction, low purity, and low purity of substances such as aflatoxin and maritin

Active Publication Date: 2019-07-09
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the many types of active ingredients in the snow chrysanthemum, it is difficult to separate a single substance, and the purity of the separated substance is low, resulting in the difficulty of extracting aflatoxin and maritin, and the purity is low.

Method used

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  • Method for extracting flavanomarein and marein in senecio cineraria
  • Method for extracting flavanomarein and marein in senecio cineraria
  • Method for extracting flavanomarein and marein in senecio cineraria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Its process route is as figure 2 As shown, the specific operation steps are as follows:

[0067] (1) Extract the dried snow chrysanthemum inflorescence with 55% ethanol solution at 47° C. for 2.5 hours under reflux to obtain the alcohol extract of snow chrysanthemum.

[0068] (2) After dissolving 10 g of chrysanthemum alcohol extract in 200 ml of water, extract with equal volume of ethyl acetate, and collect the water layer part; then use equal volume of ethyl acetate to extract the water layer part, and then collect the water layer to obtain raffinate;

[0069] (3) Extract the raffinate with an equal volume of n-butanol, and collect the organic layer; then use an equal volume of n-butanol to extract the organic layer, and then collect the organic layer, that is, the n-butanol portion, to obtain an extract.

[0070] (4) Concentrate the extract under reduced pressure to make an extract, load the sample by wet method, and perform polyamide column chromatography (polyam...

Embodiment 2

[0081] Its process route is as figure 2 As shown, the specific operation steps are as follows:

[0082] (1) Extract the dried snow chrysanthemum inflorescence with 55% ethanol solution at 47° C. for 2.5 hours under reflux to obtain the alcohol extract of snow chrysanthemum.

[0083] (2) After dissolving 70g of chrysanthemum alcohol extract in 1000ml of water, extract with equal volume of ethyl acetate, collect the water layer part; then use equal volume of ethyl acetate to extract the water layer part, and then collect the water layer to obtain raffinate;

[0084] (3) Extract the raffinate with an equal volume of n-butanol, and collect the organic layer; then use an equal volume of n-butanol to extract the organic layer, and then collect the organic layer, that is, the n-butanol portion, to obtain an extract.

[0085] (4) Concentrate the extract under reduced pressure to make an extract, load the sample by wet method, and perform polyamide column chromatography (polyamide 3...

Embodiment 3

[0094] Its process route is as figure 2 As shown, the specific operation steps are as follows:

[0095] (1) Extract the dried snow chrysanthemum inflorescence with 55% ethanol solution at 47° C. for 2.5 hours under reflux to obtain the alcohol extract of snow chrysanthemum.

[0096] (2) After dissolving 150g chrysanthemum alcohol extract in 2000ml water, extract with equal volume of ethyl acetate, collect the water layer part; then use equal volume of ethyl acetate to extract the water layer part, then collect the water layer to obtain raffinate;

[0097] (3) Extract the raffinate with an equal volume of n-butanol, and collect the organic layer; then use an equal volume of n-butanol to extract the organic layer, and then collect the organic layer, that is, the n-butanol portion, to obtain an extract.

[0098] (4) Concentrate the extract under reduced pressure to make an extract, load the sample by wet method, and perform polyamide column chromatography (polyamide 600g, 60-1...

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Abstract

The invention provides a method for extracting flavanomarein and marein in senecio cineraria. The method for extracting the flavanomarein and the marein in the senecio cineraria includes the steps that (1), the senecio cineraria chrysanthemum is subjected to reflux extraction with an ethanol solution, and senecio cineraria alcohol extract is obtained; (2), after the senecio cineraria alcohol extract is dissolved in water, the material is extracted with ethyl acetate twice, a water layer is collected, and raffinate is obtained; (3), the raffinate is extracted with n-butyl alcohol twice, an organic layer is collected, and extract liquor is obtained; (4), after the extract liquor is prepared into extract, wet process sample loading is carried out, and polyamide column chromatography is carried out; (5), eluant prepared from 90% of a phase A (water) and 10% of a phase B (ethyl alcohol) for elution is collected, rotary evaporation is carried out, and the flavanomarein is obtained; (6), eluant prepared from 60% of a phase A (water) and 40% of a phase B (ethyl alcohol) for elution is collected, rotary evaporation is carried out, and the marein is obtained. According to the method for extracting the flavanomarein and the marein in the senecio cineraria, by means of extraction and polyamide column chromatography, the flavanomarein and the marein can be effectively extracted, and high purity is achieved.

Description

technical field [0001] The invention belongs to the technical field of flavonomaside, and in particular relates to a method for extracting flavonomaside and maritin from snow chrysanthemum. Background technique [0002] Kunlun snow chrysanthemum (Coreopsis tinctoria) scientific name two-color coreopsis is an annual herb of the genus Coreopsis (Coreopsis) in the Compositae family. Coreopsis bicolor is an ornamental plant native to North America. After being successfully introduced in my country, it has been planted on a large scale in Xinjiang. It is commonly known as snow chrysanthemum. It is used as scented tea in the local area and used by Uighur folks to prevent cardiovascular diseases. In Portugal there is a tradition of drinking two cups of hot coreopsis tea a day to control diabetes. Two-color coreopsis is called snow chrysanthemum, Kunlun snow chrysanthemum, Kunlun blood chrysanthemum, alpine snow chrysanthemum, etc. in Xinjiang, and it has gradually become popular a...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08C07H17/04
CPCC07H15/203C07H1/08C07H17/04
Inventor 毛新民李新霞张永威王敬伟许芳李心雨杨婷冉峥马小芬张旭华宋志鹏鲁娜阿力腾布鲁克辛家良
Owner XINJIANG MEDICAL UNIV
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