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Processing method of high-quality bombyx mori linnaeus

A processing method and technology for dead silkworms, applied in the field of processing high-quality dead silkworms, can solve problems such as difficulty in ensuring the quality of dead silkworms is stable and controllable, lack of comparative evaluation, etc., and achieve the effects of high quality, strong operability, and good appearance and shape

Pending Publication Date: 2019-07-09
SICHUAN GUANGYUAN MINJIANG CHINESE HERBAL MEDICINE PLANTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, it is often used in combination with various methods, lacking comparative evaluation, and it is difficult to ensure stable and controllable quality of silkworms

Method used

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  • Processing method of high-quality bombyx mori linnaeus
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  • Processing method of high-quality bombyx mori linnaeus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Feeding method before embodiment 1 inoculation

[0025] 1 Experimental materials

[0026] 1.1 Source of material

[0027] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), and chlorinated lime for silkworms (purchased from Nanchong City, Sichuan Province) Taoyuan Biochemical Research Company), Canan Wang (purchased from Taoyuan Biochemical Research Company, Nanchong City, Sichuan Province), Pyocanling (purchased from Taoyuan Biochemical Research Company, Nanchong City, Sichuan Province), and Silkworm Fly (purchased from Zhejiang University Wuzhou Pharmaceutical Co., Ltd. company), Tween-80.

[0028] 1.2 Instruments and reagents

[0029] Agilent 1200HPLC high performance liquid chromatograph (Agilent Corporation of the United States); KQ-500DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); DZKW-4 ...

Embodiment 2

[0048] Embodiment 2 Bombyx mori inoculation method

[0049] 1 Experimental materials

[0050] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.

[0051] 2 Experimental design

[0052] 2.1 Screening of inoculation methods

[0053] Healthy fifth-instar silkworm larvae were selected for inoculation. Set up a blank group and an experimental group. The blank group is not inoculated, and the experimental group adopts 3 inoculation methods, method 1 (infiltration), method 2 (spray), and method 3 (feeding), with 3 repetitions in each group, a total of 12 treatment areas , the operation details are shown in Table 1. After inoculation, the ambient temperature is 24-28°C, the humidity is 90±5%RH, and the amount of mulberry is controlled after 2 hours. The fifth-instar silkworm is cultured at a temperature of 26±2°C, a hu...

Embodiment 3

[0077] Embodiment 3 Bombyx mori inoculation time selection method

[0078] 1 Experimental materials

[0079] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.

[0080] 2 Experimental design

[0081] 2.1 Preliminary study on inoculation time

[0082] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 2h, 4h, and 6h of silkworm emergence, with 3 replicates in each group, and a total of 12 treatment areas. Feeding temperature is 26±2°C, humidity is 75±5%RH, light / dark (10:14) environment, and the amount of mulberry feeding is based on full satiety.

[0083] 2.2 Optimization of inoculation time

[0084] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 8h, 16h, 24h, and 32h of...

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Abstract

The invention provides a processing method of high-quality bombyx mori linnaeus. The processing method comprises the following steps of a, taking fresh bombyx mori linnaeus which is dead due to infection of beauveria assiana (Bals.)Vuillant, wherein the infection mode involves artificial breeding infection or natural infection; b, harvesting, wherein the bombyx mori linnaeus is harvested within 24-48 hours after 80% of the bombyx mori linnaeus is dead; c, conducting pretreatment, wherein bombyx mori linnaeus bodies are spread flat, and subjected to ventilation and dehydration treatment for atleast 6 hours for ossification; d, conducting processing, wherein two-section-type circular drying is adopted for scale production, hot-air drying is conducted for 2 hour at 80 DEG C, and after dehydration, low-temperature drying is conducted at 60 DEG C; drying in the sun or drying in the shade is adopted for production site preliminary processing. The processing method is high in operability, the cost is reduced, the method is suitable for scale production and processing of the bombyx mori linnaeus, and the quality is high.

Description

technical field [0001] The invention relates to a processing method of high-quality silkworm. Background technique [0002] Bombyx mori is derived from the dry body of Bombyx mori Linnaeus 4-5 instar larvae infected with Beauveria assiana (Bals.) Vuillant, which is a typical animal drug. Fibrinolytic, anti-cancer, lipid-lowering, cosmetic and other pharmacological effects. With the in-depth research on the pharmacological components and functions of Bombyx mori, the application will become more and more extensive, and it has extremely high medicinal value and economic value. The CFDA announced the results of random inspections in 2017, and the unqualified rate of silkworms ranked 11th. The main quality problems are traits, insects, mildew, etc., and the appearance of silkworms is closely related to the processing method. [0003] Application number: 201510431732.0, Invention name: A method for cultivating silkworms, which discloses a method for cultivating silkworms, inclu...

Claims

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Application Information

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IPC IPC(8): A61K35/64
CPCA61K35/64
Inventor 何刚李永川肖禾刘文武
Owner SICHUAN GUANGYUAN MINJIANG CHINESE HERBAL MEDICINE PLANTING CO LTD
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