Processing method of high-quality bombyx mori linnaeus
A processing method and technology for dead silkworms, applied in the field of processing high-quality dead silkworms, can solve problems such as difficulty in ensuring the quality of dead silkworms is stable and controllable, lack of comparative evaluation, etc., and achieve the effects of high quality, strong operability, and good appearance and shape
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Embodiment 1
[0024] Feeding method before embodiment 1 inoculation
[0025] 1 Experimental materials
[0026] 1.1 Source of material
[0027] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), and chlorinated lime for silkworms (purchased from Nanchong City, Sichuan Province) Taoyuan Biochemical Research Company), Canan Wang (purchased from Taoyuan Biochemical Research Company, Nanchong City, Sichuan Province), Pyocanling (purchased from Taoyuan Biochemical Research Company, Nanchong City, Sichuan Province), and Silkworm Fly (purchased from Zhejiang University Wuzhou Pharmaceutical Co., Ltd. company), Tween-80.
[0028] 1.2 Instruments and reagents
[0029] Agilent 1200HPLC high performance liquid chromatograph (Agilent Corporation of the United States); KQ-500DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); DZKW-4 ...
Embodiment 2
[0048] Embodiment 2 Bombyx mori inoculation method
[0049] 1 Experimental materials
[0050] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.
[0051] 2 Experimental design
[0052] 2.1 Screening of inoculation methods
[0053] Healthy fifth-instar silkworm larvae were selected for inoculation. Set up a blank group and an experimental group. The blank group is not inoculated, and the experimental group adopts 3 inoculation methods, method 1 (infiltration), method 2 (spray), and method 3 (feeding), with 3 repetitions in each group, a total of 12 treatment areas , the operation details are shown in Table 1. After inoculation, the ambient temperature is 24-28°C, the humidity is 90±5%RH, and the amount of mulberry is controlled after 2 hours. The fifth-instar silkworm is cultured at a temperature of 26±2°C, a hu...
Embodiment 3
[0077] Embodiment 3 Bombyx mori inoculation time selection method
[0078] 1 Experimental materials
[0079] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.
[0080] 2 Experimental design
[0081] 2.1 Preliminary study on inoculation time
[0082] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 2h, 4h, and 6h of silkworm emergence, with 3 replicates in each group, and a total of 12 treatment areas. Feeding temperature is 26±2°C, humidity is 75±5%RH, light / dark (10:14) environment, and the amount of mulberry feeding is based on full satiety.
[0083] 2.2 Optimization of inoculation time
[0084] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 8h, 16h, 24h, and 32h of...
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