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MiR-125b-2-3p as molecular marker for differential diagnosis of renal cancer subtypes and use thereof in tumor metastasis

A technology of molecular markers and differential diagnosis, applied in the fields of application, microbes, and plant gene improvement, can solve problems such as unclear relationship, incomplete understanding of the pathogenesis of renal cancer subtypes, and no molecular markers of renal cancer subtypes

Inactive Publication Date: 2019-07-05
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether miR-125b-2-3p is involved in the development of RCC, especially the relationship with RCC subtypes is unclear
[0005] At present, the pathogenesis of renal cancer subtypes is not completely clear, and there are no effective molecular markers for the diagnosis and treatment of renal cancer subtypes in clinical practice. Finding new molecular markers is of great importance for the mechanism research and clinical application of renal cancer subtypes. Significance

Method used

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  • MiR-125b-2-3p as molecular marker for differential diagnosis of renal cancer subtypes and use thereof in tumor metastasis
  • MiR-125b-2-3p as molecular marker for differential diagnosis of renal cancer subtypes and use thereof in tumor metastasis
  • MiR-125b-2-3p as molecular marker for differential diagnosis of renal cancer subtypes and use thereof in tumor metastasis

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Embodiment 1

[0067] Example 1: Low expression of miR-125b-2-3p is specifically related to ccRCC and its relationship with patient prognosis

[0068] All RCC and matched paracancerous tissues in this study were collected from Ningbo Urological and Nephrological Hospital and confirmed by pathological examination. Patients informed and agreed that the samples were used in this study, and this study was approved and implemented by the Ethical Science Committee of Ningbo University.

[0069] The result is as follows:

[0070] (1) Low expression of miR-125b-2-3p is specifically related to ccRCC

[0071] To investigate the relationship between miR-125b-2-3p expression and RCC subtypes, we analyzed the expression of miR-125b-2-3p in ccRCC (66 cases), pRCC (12 cases) and chRCC (7 cases) difference. The results showed that patients with low expression of miR-125b-2-3p accounted for 53 / 66 (80.3%) of ccRCC samples, which was significantly higher than the other two (P=0.001) (Table 7). It can be se...

Embodiment 2

[0083] The role of miR-125b-2-3p in affecting the metastasis of renal clear cell carcinoma and its prognostic factors

[0084] 1. MTS experiment:

[0085] 24h after transient transfection, MTS assay was performed. Pour off the old medium, rinse with PBS once; add an appropriate amount of 0.25% trypsin, digest at 37°C until the cells retract and become rounded or the intercellular space increases, then immediately add complete medium to stop digestion; repeatedly pipette the cells to detach Wall and disperse to form a cell suspension; suck into a 15ml centrifuge tube, centrifuge at 1000r / m for 5min; pour off the supernatant, add 1ml complete medium to resuspend the cells, and count the cells; dilute the cells to 50,000 cells with complete medium / ml culture medium, then add cells to 96-well plate, 100μl cell suspension per well, make 5 duplicate holes; 37℃, 5% CO 2 , Routine culture in a saturated humidity incubator, and 20 μl of MTS reagent (Cell Titer Aqueous One Solution...

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Abstract

The present invention relates to a MiR-125b-2-3p as a molecular marker for differential diagnosis of renal cancer subtypes and a use thereof in tumor metastasis. The biomolecular marker MiR-125b-2-3prelated to genesis and development of renal clear cell carcinoma is firstly discovered. By detecting an expression of the MiR-125b-2-3p in patient tissues and blood, early diagnosis of the renal clearcell carcinoma is experimented. Besides, a molecular target for treatment of the renal clear cell carcinoma is firstly provided. The treatment by the targeting and molecular marker in the metastaticrenal clear cell carcinoma has sensitivity and specificity.

Description

technical field [0001] The invention belongs to the field of tumor markers, in particular to a molecular marker MiR-125b-2-3p for differential diagnosis of kidney cancer subtypes and its application in tumor metastasis. Background technique [0002] Renal cell carcinoma (RCC), originating from the epithelium of the urinary tubules of the renal parenchyma, is the most common and fatal renal malignancy, accounting for 90% of all renal tumors. RCC is a heterogeneous disease comprising a spectrum of malignancies with marked genetic and / or epigenetic differences and clinical features [2,3] , including clear cell RCC (ccRCC), papillary RCC (pRCC) and chromophobe RCC (chRCC) [4,5] . At present, surgical resection is still the most important method for the treatment of renal cell carcinoma, but the treatment effect is not good. At the same time, because different RCC subtypes have obvious differences in genetics and epigenetics, the prognosis of patients with different RCC subtyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11C12N15/867
CPCC12Q1/6886C12N15/86C12N2740/15043C12Q2600/156C12Q2600/118C12Q2600/178
Inventor 王萍孟祥余马琪陈一勇
Owner NINGBO UNIV
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