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Preparation method and application of Brevundimonas naejangsanensis enzyme agent to efficiently degrade dimethachlon

A high-efficiency degrading bacteria and degrading enzyme technology, applied in the field of biotechnology degradation of pesticide residues, can solve problems such as still sterile nuclear net biodegradation, and achieve the effects of environmental friendliness, remarkable effect and good quick effect.

Inactive Publication Date: 2019-07-05
GUIZHOU UNIV
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  • Claims
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Problems solved by technology

To date, no research has been done on the biodegradation of sclerotinia in agricultural products

Method used

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  • Preparation method and application of Brevundimonas naejangsanensis enzyme agent to efficiently degrade dimethachlon
  • Preparation method and application of Brevundimonas naejangsanensis enzyme agent to efficiently degrade dimethachlon

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Embodiment 1

[0017] 1. Enzyme preparation:

[0018] 1) Bacterial suspension preparation: Add 2 mL of sterile phosphate buffer under aseptic conditions and shake in parallel in the culture dish cultured for 48 hours, and gently scrape off the colonies on the surface of the medium with an inoculation loop to form a crude bacterial suspension. liquid, and transferred it to a liquid fermentation medium (maltose 7g / L, yeast extract 6g / L, NaCl 3g / L) at a pH of 7.5-8, 30-35°C, and 150-200rpm for 48 hours. Immediately centrifuge the fermented broth taken out at 10000r / min (3-4°C) for 10min, collect the wet cells, and wash them three times with sterile phosphate buffered saline. Then mix the sterile phosphate buffer solution with the wet cells, and control the number of cells to be ≥6×10 8 cfu / mL. The prepared bacterial suspension was stored at 3-4°C for later use.

[0019] 2) Extraction of free enzymes: Ultrasonic crushing was performed on the bacterial suspension prepared in step 1) to extract...

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Abstract

The invention discloses a preparation method and application of a Brevundimonas naejangsanensis enzyme agent to efficiently degrade dimethachlon. Brevundimonas naejangsanensis CCTCC M 2017738 is usedherein as a strain; the Brevundimonas naejangsanensis enzyme agent is prepared by subjecting the strain to suspension propagation and performing degradation enzyme agent ultrasonic crushing and extraction. The enzyme agent herein is applicable to the efficient degradation of dimethachlon, vinclozolin and iprodione in soil, water and plants, such as rape, Chinese cabbage, Brassica campestris L. var. purpuraria, Lactuca sativa var. ramosa, Allium tuberosum, cucumber, tomato, hot chili, potato and tobacco. The enzyme agent herein has the advantages of high acting speed, good environmental friendliness, significant effect, low cost, good popularization convenience and the like.

Description

technical field [0001] The invention relates to the field of biotechnological degradation of pesticide residues, in particular to a preparation method and application of a Monas bacterium enzyme agent for efficiently degrading sclerotinia. Background technique [0002] Pesticides play an important role in the integrated management of agricultural pests, but pesticide residues and their metabolites have become serious pollutants, posing threats to human health and ecosystems. Dimetachlone, also known as Dimetachlone, has molecular formula C 10 h 7 Cl 2 NO 2 , its relative molecular mass is 244.07, and its chemical name is: N-(3,5-dichlorophenyl)succinimide. In my country, it is widely used in the prevention and control of fungal diseases such as sclerotinia, sheath blight, and red spot on rapeseed, Chinese cabbage, laver moss, lettuce, leek, cucumber, tomato, pepper, potato, tobacco and other crops. Despite its promising bactericidal activity, sclerotinia is currently ba...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/00C02F3/34B09C1/10C12R1/01C02F101/38C02F101/34
CPCC12N1/20C12N9/00C02F3/342B09C1/10C02F2101/306C02F2101/34C02F2101/38
Inventor 吴小毛张承李姣红龙友华李荣玉王秋萍代园凤黄文源
Owner GUIZHOU UNIV
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