A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof

A chitosan nanometer, bronchial asthma technology, applied in the field of biomedicine, can solve the problems of inability to reduce airway reactivity, reduce airway sensitivity, etc., achieve easy grasp of operation methods, reduce airway sensitivity, and reduce airway sensitivity. Effects of Inflammatory Response

Active Publication Date: 2020-08-04
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it does not reduce airway responsiveness and airway sensitivity

Method used

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  • A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof
  • A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof
  • A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Preparation of DGKα gene and OVA antigen composite chitosan nanoparticles (CS-DGKα-OVA)

[0045] 1. Construct a vector expressing DGKα gene: synthesize the fusion gene of mouse IL-2 signal peptide sequence and mouse DGKα gene with full gene, and clone into pEGFP-N3 vector to obtain DGKα plasmid. Sequencing confirmed that the IL-2 signal peptide and mouse DGKα gene sequence were completely consistent with IL-2 (NM_008366.3) and DGKα gene (NM_016811.2) in GenBank. Because of the signal peptide sequence, after the DGKα gene is transfected into the cell, the cell can express the DGKα protein and secrete it outside the cell.

[0046] 2. Preparation of CS-DGKα-OVA:

[0047] 2.1 Dissolve chitosan (molecular weight: 100,000, DD: 95%) in 1% acetic acid aqueous solution to prepare a chitosan acetic acid solution with a concentration of 1.5 mg / mL, adjust the pH to 5.5-5.7 with NaOH, and sterile 0.22 μm Membrane filtration.

[0048] 2.2 Dissolve the DGKα plasmid in a sterile 0.22μm membr...

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Abstract

The invention relates to the field of biological medicine, in particular to composite chitosan nano particles for treating bronchial asthma and application thereof. The composite chitosan nano particles are particles formed by using CS (chitosan) to wrap DGKalpha gene(diacylglycerol kinase alpha gene) expression vectors and OVA (ovalbumin), can evidently lower OVA specific IgE antibody level in serum and bronchial inflammatory reaction and can evidently lower asthma airway responsiveness and airway sensitivity, and accordingly the particles are promising in application prospect in the preparation of drugs and / or health-care products for treating and / or preventing bronchial asthma.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a composite chitosan nanoparticle for treating bronchial asthma and its application. Background technique [0002] Bronchial asthma is a chronic airway allergic inflammatory disease characterized by the involvement of a variety of inflammatory cells and the infiltration of airway eosinophils. After the human body inhales allergens, the airway antigen presenting cells take up and present the allergens to T cells, which promotes the excessive activation and proliferation of T cells, which initiates the occurrence of asthma. Of which CD4 + Excessive activation of T cells is thought to play an important role in the pathogenesis of asthma. CD4 + After activation of T cells, the differentiation into Th2 cells increases, and the overactivation of Th2 cells leads to an immune response dominated by Th2 response, which eventually leads to the accumulation of inflammatory cells such as eosinophils in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K9/50A61K47/36A61P11/06A61K39/35
CPCA61K9/5036A61K39/35A61K48/0033A61P11/06A61K2300/00
Inventor 王彦毕玉田郭东霖黄丽云王斌
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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