Liquid chromatography tandem mass spectrometry method for detecting content of 17-hydroxyprogesterone in blood

Abstract

The liquid chromatography tandem mass spectrometry method for detecting the content of 17-hydroxyprogesterone in blood comprises steps of calibrating a standard solution by a high-performance liquid chromatography tandem mass spectrometry; fitting a standard curve equation y=a*x+b; taking a blood sample; after the blood to be detected is processed, detecting the blood to be detected by also usingthe high-performance liquid chromatography tandem mass spectrometer to obtain the value y of the blood to be detected; substituting the value y of the blood to be detected into the standard curve equation, and calculating the relative concentration x of 17-hydroxyprogesterone in the blood sample to be detected, wherein the concentration of an internal standard substance working fluid is known, andaccordingly the concentration of 17-hydroxyprogesterone in the blood to be detected in the sample is calculated. The serum of the present invention is subjected to nitrogen-blowing redissolving injection after extraction centrifugation. The method is simple and rapid in detection process, low in experiment cost, and contributes to monitoring the concentration of 17-hydroxyprogesterone in a patient during clinical treatment.

Description

Technical field [0001] The present invention relates to the technical field of determining the content of hormones in clinical blood, in particular to a method for detecting the concentration of 17-hydroxyprogesterone in the blood. Background technique [0002] 17-hydroxyprogesterone (17-hydoxy progesterone, 17-OHP), the molecular formula is C 21 H 30 O 3 , The specific chemical structure is as follows figure 1 Shown. The reference interval for the biological concentration of 17-hydroxyprogesterone: 0.04-10.0ng / mL. 17 - Hydroxyprogesterone is produced by the adrenal cortex and gonads, and its progesterone activity is very low. 17-OHP is 21-hydroxylated to produce Cortisol precursor compound S (CpS). 17-OHP has circadian rhythm changes consistent with adrenal cortisol. The 17-OHP concentration of adult women of childbearing age varies with the menstrual cycle, and the luteal phase is higher than the follicular phase. The fetus, placenta and adrenal glands can produce a large a...

AI Technical Summary

Problems solved by technology

High performance liquid chromatography is accurate in quantification and has good reproducibility, but there are still problems such as complicated pretreatment and long analysis time, so it is not suitable for clinical testing

Clinical radioimmunoassay (RIA) is commonly used to measure 17-OHP level. This method has short analysis time, strong characteristics, and good economic benefits, but it is inevitable that there are endogenous structural analogs that will affect the test results.

Liquid chromatography tandem mass spectrometry has the advantages of high sensitivity, strong specificity, and short analysis time, but it is costly and has high requirements for personnel and the environment

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