Self-driven microfluidic chip and method for using same

Abstract

The invention provides a self-driven microfluidic chip and a method for using the same. The self-driven microfluidic chip includes a substrate and a packaging sheet. A sample inlet, a sample outlet, aplurality of reaction chambers and one or more main channels are formed between the substrate and the packaging sheet. The sample inlet and the sample outlet can be communicated with the outside, themain channels are communicated with the sample inlet and the sample outlet, and liquid inlet channels and a liquid outlet channel are disposed between each of the reaction chambers and one main channel. The inner surfaces of the main channel, the reaction chambers, the liquid inlet channels and the liquid outlet channel located on the substrate and / or the packaging sheet are hydrophilic surfaces,the cross sectional area of the liquid inlet channel is smaller than that of the liquid outlet channel, and in the liquid flow direction in the main channel, the liquid inlet channels are located onthe front side of the liquid outlet channel. After a to-be-tested sample solution enters the reaction chamber, an oil phase reagent can be led into the main channel to seal the liquid inlet channels and the liquid outlet channel. The self-driven microfluidic chip can achieve self-driven flowing sample loading of the to-be-tested sample solution without an external driving device.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a self-driven microfluidic chip and a method for using the same. Background technique [0002] In the past 20 years, there have been more than 2.2 million new cancer cases in my country every year. At the same time, the new cases are still increasing at a rate of 3%-5% every year. Cancer has become the number one cause of death affecting the life and health of our people. The occurrence of cancer needs to go through several steps such as gene mutation, cancer cell generation, tumor formation and finally metastasis and spread. The current methods of cancer diagnosis mainly include pathological examination and imaging examination. More accurate detection results cannot be obtained until the tumor grows to a certain size and the cancer develops in the middle and late stages, which often misses the best treatment time for patients. If the detection of mutated genes can be realized ...

AI Technical Summary

Problems solved by technology

In the related manipulation of microfluidic chips, equipment such as syringes or air pumps and electric pumps are usually used to drive reagents to flow in the chip, which makes the operation of the chip complicated and requires corresponding instruments to realize the function.

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