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Self-driving microfluidic chip and method of using the same

A microfluidic chip, self-driven technology, applied in fluid controllers, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as complex chip operations, achieve low experimental costs, and good anti-fusion effects

Active Publication Date: 2021-07-27
SHENZHEN SHINEWAY HI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the related manipulation of microfluidic chips, equipment such as syringes or air pumps and electric pumps are usually used to drive reagents to flow in the chip, which makes the operation of the chip complicated and requires corresponding instruments to realize the function.

Method used

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  • Self-driving microfluidic chip and method of using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This implementation relates to a self-driven microfluidic chip, which is used to accommodate the sample solution to be tested, so as to perform various detection operations such as real-time fluorescent quantitative PCR detection or digital PCR detection on the sample solution to be tested. Such as figure 1 As shown in , the self-driven microfluidic chip includes a substrate 1 and an encapsulation sheet 2, at least one of the substrate 1 and the encapsulation sheet 2 is a colorless and transparent material, and the colorless and transparent material should also be self-low fluorescent , and the low fluorescence characteristic of this embodiment is generally that under the irradiation of the excitation light wavelength of the fluorescent probes used in PCR reagents such as FAM, HEX, VIC, CY3, TAMRA, ROX, CY5, etc., the excited fluorescence intensity of the material is less than 10e Fluorescence intensity of -8mol / L fluorescein. Due to the low fluorescence characteristic...

Embodiment 2

[0057] This implementation involves a self-driven microfluidic chip, which has roughly the same structure as the self-driven microfluidic chip in Example 1, the difference lies in the combination figure 2 and image 3 As shown in , in this embodiment, there are multiple main channels 13, and a waste liquid pool 17 is provided between these main channels 13 and the sample outlet 12, and the structure of the reaction chamber 14 is square. The solution to be tested or the oil phase reagent in each main channel 13 can enter the waste liquid pool 17 and then flow to the sample outlet 12 .

[0058] In addition, when the self-driven microfluidic chip of this embodiment is used for real-time fluorescent quantitative PCR detection or digital PCR nucleic acid detection, its specific method of use includes the following steps, and the sample solution to be tested hereinafter can be commercialized HBV DNA fragments. When the self-driven microfluidic chip of the first embodiment is used...

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Abstract

The invention provides a self-driven microfluidic chip and its use method. The self-driven microfluidic chip of the present invention includes a substrate and a packaging sheet, and a sample inlet, a sample outlet, a plurality of The reaction chamber and one or more main channels; the sample inlet and the sample outlet can communicate with the outside world, the main channel is connected with the sample inlet and the sample outlet, and there is a liquid inlet channel and a channel between each reaction chamber and a main channel. The liquid outlet channel; the inner surface of the main channel, the reaction chamber, the liquid inlet channel and the liquid outlet channel located on the substrate and / or the packaging sheet is a hydrophilic surface, and the cross-sectional area of ​​the liquid inlet channel is smaller than the cross-sectional area of ​​the liquid outlet channel, In the direction of liquid flow in the main channel, the liquid inlet channel is located at the front side of the liquid outlet channel; after the sample solution to be tested enters the reaction chamber, an oil phase reagent can be introduced into the main channel to seal the liquid inlet channel and the liquid outlet channel. The self-driven microfluidic chip of the present invention can realize the self-driven flow loading of the sample solution to be tested without setting external driving equipment.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a self-driven microfluidic chip and a method for using the same. Background technique [0002] In the past 20 years, there have been more than 2.2 million new cancer cases in my country every year. At the same time, the new cases are still increasing at a rate of 3%-5% every year. Cancer has become the number one cause of death affecting the life and health of our people. The occurrence of cancer needs to go through several steps such as gene mutation, cancer cell generation, tumor formation and finally metastasis and spread. The current methods of cancer diagnosis mainly include pathological examination and imaging examination. More accurate detection results cannot be obtained until the tumor grows to a certain size and the cancer develops in the middle and late stages, which often misses the best treatment time for patients. If the detection of mutated genes can be realized ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00C12Q1/6851
CPCB01L3/502769B01L7/52B01L2200/0689B01L2300/0636B01L2300/0816B01L2300/0864B01L2400/0406B01L2200/142B01L2300/087B01L2200/0621
Inventor 温维佳高一博宋祺
Owner SHENZHEN SHINEWAY HI TECH CO LTD
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