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Method for creating breeding component of wheat lasting in resistance to powdery mildew based on multi-gene pyramiding

A powdery mildew resistance and multi-gene technology is applied in the direction of plant gene improvement, botanical equipment and methods, applications, etc. It can solve the problems of powdery mildew resistance gene resistance loss and other problems, and achieve comprehensive agronomic traits, powdery mildew stability, and ripening. good effect

Pending Publication Date: 2019-06-25
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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Problems solved by technology

[0003] Purpose of the invention: In order to solve the problem of the loss of resistance of a single powdery mildew resistance gene in the prior art, the present invention proposes a multi-gene aggregation-based creation of powdery mildew resistance with long-lasting resistance against factors such as powdery mildew hazards in actual production Method for diseased wheat breeding elements

Method used

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  • Method for creating breeding component of wheat lasting in resistance to powdery mildew based on multi-gene pyramiding
  • Method for creating breeding component of wheat lasting in resistance to powdery mildew based on multi-gene pyramiding
  • Method for creating breeding component of wheat lasting in resistance to powdery mildew based on multi-gene pyramiding

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Embodiment 1

[0021] combine figure 1 Shown, the method for cultivating powdery mildew resistant wheat based on multigene aggregation comprises the following steps:

[0022] (1) Select Zhenmai 9 as the female parent with medium early maturity, good ripeness, excellent comprehensive traits, and pm21 as the female parent, and Ningmai 23 (pm8) with high and stable yield as the male parent, and sexually cross the two to obtain the hybrid F 1 , for F 1 Carry out artificial inoculation powdery mildew (E26, E31 equivalence mixture), screen disease-resistant material and self-cross to obtain F 2 ;

[0023] (2) For key combinations and limited populations, use the specific molecular marker WS-1F / R closely linked to pm21 and the rye chromatin-specific primer AF1 / AF4 (pm8) to F 2 Detect to obtain strains that polymerize pm21 and pm8 at the same time; for F 2 The combination of artificial inoculation of powdery mildew for resistance identification and molecular marker-assisted selection is used to ...

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Abstract

The invention discloses a method for creating a breeding component of wheat lasting in resistance to powdery mildew based on multi-gene pyramiding. The method comprises the steps that according to a breeding target, parent materials are subjected to pyramiding breeding, and the wheat lasting in resistance to powdery mildew is obtained, wherein the breeding target at least comprises the polymeric pm 21, polymeric pm8, polymeric pm4 and polymeric pm38 and the resistance to powdery mildew, and the parent materials are Zhen wheat No.9, Ning wheat 23, Yang wheat 11 and Pingyuan 50. By selecting theparents with different genes resistant to powdery mildew, the four gene progenies including the polymeric pm 21, the polymeric pm8, the polymeric pm4 and the polymeric pm38 are created, and the wheatwhich is lasting in resistance to powdery mildew, high in tillering ability, short in stalk, resistant to lodging, wide in applicability, and high and stable in yield is obtained. The wheat has the advantages of being large in ear and kernel, good in mature appearance, outstanding in comprehensive agronomic trait and the like; the cultured wheat resistant to powdery mildew is stable in resistanceto powdery mildew, lasting in resistance, high and stable in yield and outstanding in comprehensive agronomic trait, and the problem of resistance loss of a single resistance gene is solved.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, and relates to a method for creating durable powdery mildew-resistant wheat breeding elements based on polygene aggregation. Background technique [0002] Since the late 1970s, wheat powdery mildew has become an important disease of wheat production in my country. In addition, the large-scale promotion and planting of varieties containing single disease-resistant genes pm8 and pm21, as well as the large number of physiological races of powdery mildew and rapid mutation, have caused the severity of wheat powdery mildew to increase. In recent years, the incidence of powdery mildew has remained at 60,000-80,000 hectares, in order to prevent and control crop diseases every year, my country needs to invest a lot of pesticides. The use of a large number of pesticides will inevitably cause pesticide residues in food and pesticide pollution in soil and water sources, seriously threatening food and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 温明星李东升温小林陈琛郭瑞卢盼盼曲朝喜
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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