Metabolomics marker weight analysis universal method

A technology of weight analysis and general method, applied in the field of metabolomics detection, can solve the problems of correlation deviation and reduce the specificity of metabolic markers, so as to improve the discrimination ability, reduce the threshold of technical application and increase the readability.

Inactive Publication Date: 2019-06-21
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
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Problems solved by technology

However, the metabolism of organisms is a complex steady-state network system. A metabolite usually involves changes in multiple potential metabolic pathways. Although the analysis of the enrichment level of metabolic markers in known metabolic pathways in KEGG can explain some of the differences Due to the limitations of the database, the enrichment analysis results often ignore the influence of factors such as metabolic differences between species and unknown metabolic pathways.
At the same time, ignoring the correlation between metabolites and single database enrichment analysis will easily lead to bias in the evaluation of the biological effects of metabolic markers, thereby reducing the specificity of metabolic markers

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  • Metabolomics marker weight analysis universal method
  • Metabolomics marker weight analysis universal method
  • Metabolomics marker weight analysis universal method

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Embodiment 1

[0043] This embodiment relates to the screening of abnormal metabolic pathways in gestational diabetes mellitus based on the metabolic profile of umbilical cord serum, which specifically includes the following steps:

[0044] 1. Collection and pretreatment of serum samples

[0045] Umbilical cord blood was collected from 65 healthy pregnant women without obvious complications during pregnancy and 31 pregnant women with gestational diabetes mellitus and no other perinatal complications, and serum metabolites were extracted by liquid-liquid extraction. All cord blood samples were collected in Xiamen Maternal and Child Health Hospital, and all subjects signed written informed consent.

[0046] 2. Serum metabolism detection

[0047] After necessary pretreatment, the extracted serum metabolic profile was obtained by Thermo Fisher Scientific Q Exactive liquid chromatography-mass spectrometry system. The separation of metabolites uses a reverse chromatographic column, and the molec...

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Abstract

The invention provides a metabolomics marker weight analysis universal method which comprises the following steps of S1, performing metabolite correlation analysis; S2, performing network analysis fordetermining a key metabolism network; S3, performing multiple regression analysis and determining a metabolic marker; and S4, based on network degree centrality, determining importance of the metabolic marker in the key metabolism network. According to the method of the invention, by means of network analysis, importance of the metabolic marker in the key metabolism network is quantified throughnetwork degree centrality, thereby supplying an evaluation method for biological weight of the metabolic marker, improving accuracy in identifying the metabolic marker, and realizing high applicability in analyzing other metabolomics platform markers.

Description

technical field [0001] The invention relates to a general method for weight analysis of metabolomics markers, belonging to the technical field of metabolomics detection. Background technique [0002] With the continuous improvement of detection methods and data computing capabilities, omics technology based on high-throughput detection and big data analysis plays an increasingly important role in the research of multiple levels of biological genes, transcription, protein, and metabolism. . Omics technology measures and analyzes a large amount of biological information at the overall level, and expounds the potential mechanism of changes in the activities of living organisms under the influence of the environment or genes from a multi-dimensional and systematic perspective. [0003] As an important part of omics technology, metabolomics mainly studies small metabolic molecules below 1000 Da, and these small molecules can directly and accurately reflect the current physiologi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B5/00G16B20/00G16B40/00G16B50/00G06F17/18
Inventor 吴衍张洁申河清丁蕊张西
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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