Application of GPR1 (G protein coupled receptor 1) target and its antagonist in infertility-related diseases

An antagonist and disease technology, which is applied in the application field of GPR1 and its antagonists in the treatment of ovulation disorder-related diseases, and can solve the problems of not protecting patients with premature ovarian failure, etc.

Active Publication Date: 2019-06-21
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no clearly proven effective treatments to restore or protect ovarian function in patients with premature ovarian failure

Method used

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  • Application of GPR1 (G protein coupled receptor 1) target and its antagonist in infertility-related diseases
  • Application of GPR1 (G protein coupled receptor 1) target and its antagonist in infertility-related diseases
  • Application of GPR1 (G protein coupled receptor 1) target and its antagonist in infertility-related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Interfering with the gene expression of GPR1, or antagonizing the function of GPR1, can effectively alleviate the symptoms of polycystic ovary syndrome in experimental mice caused by DHEA.

[0047] In this example, it is verified that interfering with the gene expression of GPR1, or antagonizing the effect of GPR1, can effectively alleviate the symptoms of experimental mouse polycystic ovary syndrome caused by DHEA. Among them, an experimental mouse polycystic ovary syndrome model induced by hyperandrogen DHEA was established. However, compared with wild-type mice, the symptoms of polycystic ovary syndrome in GPR1 knockout mice were significantly alleviated, including: increased serum estrogen levels, and corpus luteum production on the ovaries.

[0048] details as follows:

[0049] (1) DHEA experimental mouse polycystic ovary syndrome model

[0050] GPR1 knockout mice in C57BL / 6J background were purchased from Deltagen Co., Ltd. and provided by Dr. ZABLE's...

Embodiment 2

[0066] Example 2: GPR1 regulates the expression of estrogen-related synthetase caused by DHEA through the mTOR signaling pathway

[0067] C57BL / 6J wild-type female mice were from Guangdong Medical Experimental Animal Center.

[0068] Animals were housed in a vivarium with a 12-h light-dark cycle at constant temperature and humidity. Feed and water were available ad libitum. All animal procedures were performed in accordance with procedures approved by the Animal Welfare Ethics Committee.

[0069] The mice were intraperitoneally injected with 5IU PMSG (pregnant horse serum gonadotropin) at 21 days, and then 5IU hCG (human chorionic gonadotropin) was injected intraperitoneally after 48 hours. After 7-12 hours, the mice were killed, the ovaries were removed, and the follicles were punctured. Pass through a 70um sieve three times to remove oocytes, centrifuge at 1000g for 5 minutes, discard the supernatant, and resuspend in a serum-free DMEM F12+3%BSA+ITS incubator at 37°C, 5%CO...

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Abstract

The invention provides application of GPR1 (G protein coupled receptor 1) target and its antagonist in infertility-related diseases. It is determined herein that the chemerin/GPR1 system is a novel target to regulate polycystic ovarian syndrome, is closely associated with mTOR signal pathway associated with the activation of primordial follicles, and may involve in the process of regulating the activation of primordial follicles. The invention also provides application GPR1 gene or GPR1 protein in screening drugs and diagnostic targets to treat or prevent ovulatory obstacle and related diseases, and discloses application of a GPR1 gene or GPR1 protein antagonist in the prevention or treatment of ovulatory obstacle and related diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of GPR1 and its antagonist in the treatment of diseases related to ovulation disorder. Background technique [0002] Infertility is a common reproductive disorder that affects at least 10%-15% of couples of reproductive age. There are many factors that can cause infertility, such as organic infertility caused by organ lesions, anovulatory infertility caused by ovarian function decline or premature ovarian failure, ovulatory infertility caused by ovulation disorders, etc., which are harmful to women's health and family. Both happiness and well-being are seriously jeopardized, therefore, the prevention and treatment of female infertility is of great significance. [0003] (1) Organic infertility [0004] Organic infertility refers to infertility caused by pathological and anatomical changes in the genitals, such as inflammation of the reproductive system, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883A61K45/00A61P15/08
Inventor 张键杨雅莉孙立峰肖天霞汪宝蓓
Owner SHENZHEN INST OF ADVANCED TECH
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