Fluid culture medium and method for isolated-culturing mycoplasma gallisepticum using same

Abstract

The invention discloses a fluid culture medium. The fluid culture medium is prepared by mixing a basic culture medium and an auxiliary culture medium. The basic culture medium contains the following components by mass concentration (g / L): 3-7 g / L of sodium chloride, 0.3-0.6 g / L of potassium chloride, 1.5-3.5 g / L of sodium phosphate dibasic dodecahydrate, 0.1-0.4 g / L of magnesium sulfate heptahydrate, 0.1-0.2 g / L of potassium dihydrogen phosphate, 10-15 g / L of glucose, 6-8 g / L of lactoalbumin hydrolysate and 6-7 g / L of yeast extract powder; and the auxiliary culture medium contains the following components by volume concentration (mL / L): 40-80 mL / L of fetal calf serum, 1-2 mL / L of penicillin, 2-3 mL / L of phenol red, 40-80 mL / L of a DMEM high glucose culture medium, 8-10 mL / L of arginine, 8-10 mL / L of cysteine and 8-10 mL / L of coenzyme I. The invention further discloses a preparation method for the culture medium and a method for isolated-culturing mycoplasma gallisepticum using the culture medium. The fluid culture medium is capable of providing the more excellent growing conditions, accelerating metabolism, advantageously shortening isolation time of the mycoplasma gallisepticum and improving an isolating rate.

Description

technical field [0001] The invention relates to the technical field of veterinary biology, in particular to a liquid culture medium and a method for isolating and culturing Mycoplasma gallisepticum using it. Background technique [0002] At present, the diseases with latent infection in the breeding industry are mainly mycoplasma gallisepticum, which is a kind of Mycoplasma gallisepticum. The common pathogenic mycoplasma in poultry can be classified into three categories: Mycoplasma gallisepticum, Mycoplasma synovialis and Mycoplasma turkey, among which Mycoplasma gallisepticum is the most harmful to poultry farming. Mycoplasma gallisepticum infection of chickens can cause outbreaks of chronic respiratory diseases, mainly sinusitis, bronchitis and air sacculitis, accompanied by secondary symptoms. The lethality rate of Mycoplasma gallisepticum is low, which usually leads to slow growth of sick chickens, lower feed conversion rate, lower carcass quality of broiler chickens, ...

AI Technical Summary

Problems solved by technology

Clinical isolation of strains is a method of obtaining purified single colonies of target strains from miscellaneous flora through professional separation and culture techniques. The traditional method uses dissected lungs and other tissues to isolate Mycoplasma gallisepticum, which cannot guarantee the integrity of animals. And the medium used to cultivate Mycoplasma gallisepticum needs 3-7 days for each generation of culture. Since the isolated strains need multiple generations of continuous screening, a large amount of medium is consumed, which also causes problems such as long time-consuming and low isolation rate.

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