Clamp-type immunoblotting analyzer and control system thereof

Abstract

The invention provides a clamp-type immunoblotting analyzer and a control system thereof. The clamp-type immunoblotting analyzer comprises a pedestal, a sample film clamping piece, a lofting transferarea, a reaction temperature area, and a transfer unit. The transfer unit comprises a chuck and an XYZ three-axis moving mechanism. The chuck is provided with a mechanism for carrying out taking and placing of the sample film clamping piece. The XYZ three-axis moving mechanism drives the chuck to move between the lofting transfer area and each reaction temperature area. The invention also providesa reagent storage area so that an expensive reagent can be conveniently recycled and experiment cost is reduced. A new sample film can be inserted into the immunoblotting analyzer to carry out reaction at any time, and test steps and conditions of each sample film can be separately and individually set, which is suitable for test operation of laboratory personnel. Usage efficiency of the immunoblotting analyzer is greatly improved, waiting time for queuing is reduced, and a test speed is increased.

Description

technical field [0001] The invention relates to a biological test auxiliary equipment, in particular to a clamping immunoblotting analyzer and its control system. Background technique [0002] Western blot test is also called Western Blot test (Western Blot). It is an experimental method commonly used in biochemistry and immunogenetics. It transfers the total protein of cells or tissues separated by electrophoresis from the gel to the solid-phase carrier NC membrane or PVDF membrane, and then transfers the total protein of the cell or tissue on the solid-phase carrier to Proteins or polypeptides are used as antigens to immunoreact with corresponding antibodies, then react with enzymes or isotope-labeled secondary antibodies, and finally undergo substrate color development or autoradiography to detect protein components expressed by specific target genes separated by electrophoresis . In the whole immune reaction test, it is necessary to perform multiple cleaning-incubation...

AI Technical Summary

Problems solved by technology

For a group of immunoblotting experiments that take several hours, the utilization rate of the instrument is not high, and the laboratory needs to be equipped with multiple sets at the same time, which seriously occupies the experimental space and increases the purchase cost.

In addition, for some expensive and reusable primary and secondary antibody test solutions, they need to be stored at low temperature immediately after the reaction. In the existing western blot analyzers, the temperature control area is generally used to achieve temperature change, and the temperature change process is relatively slow. , time-consuming and energy-intensive

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