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Application of hsa-miR-15b-5p as molecular marker and kit

A technology of hsa-mir-15b-5p, 1.hsa-mir-15b-5p, which is applied in molecular biotechnology and its application fields, achieves the effects of good screening efficiency, alleviation of altitude sickness and large number of

Active Publication Date: 2019-06-18
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the correlation between circulating microRNAs and AMS susceptibility.

Method used

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  • Application of hsa-miR-15b-5p as molecular marker and kit
  • Application of hsa-miR-15b-5p as molecular marker and kit
  • Application of hsa-miR-15b-5p as molecular marker and kit

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Example 1 Correlation between the expression of microRNA in saliva samples and the risk of AMS

[0042] 1. Material and Specimen Collection Description

[0043] The saliva samples of AMS-susceptible persons were collected from AMS patients who entered the plateau rapidly before entering the plateau, a total of 75 cases. The saliva samples of normal people were collected from 49 cases of normal healthy people who entered the plateau rapidly before entering the plateau. The diagnosis of AMS was confirmed by the International Common Diagnostic Criteria-Lake Lewis Score. All the groups did not take any preventive medicine before taking saliva. A total of 10ml of saliva was collected from each sample in a sterile, enzyme-free 50ml centrifuge tube. The clinical case characteristics of the patients are shown in Table 1. This study has been approved by the Medical Ethics Committee of the Army Military Medical University (Third Military Medical University), and all specimens...

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Abstract

The invention relates to an application of hsa-miR-15b-5p as a microRNA marker for screening saliva of an acute plateau reaction susceptible person, wherein the microRNA is applied to preparation of areagent or a kit for screening the acute plateau reaction susceptible person, and the expression of hsa-miR-15b-5p in a saliva sample is down-regulated compared with the expression in a control. Theinvention further provides a kit for detecting hsa-miR-15b-5p molecules in saliva of a detected human plain by a real-time fluorescence quantitative PCR method, and the risk of acute plateau reactionis predicted according to the expression level of the hsa-miR-15b-5p molecules. The microRNA is used as a molecular marker, and has the advantages of strong specificity and high sensitivity; the obtaining of a detected sample is non-invasive and convenient; the microRNA is suitable for screening large-scale acute plateau reaction susceptible people in a plain.

Description

technical field [0001] The invention belongs to the field of molecular biotechnology and its application. The invention relates to an application and a kit for hsa-miR-15b-5p as a microRNA molecular marker for screening the saliva of persons susceptible to acute altitude sickness. Screen the risk of acute altitude sickness by screening the expression level of hsa-miR-15b-5p in saliva, so as to evaluate the human body's susceptibility to acute altitude sickness and reduce the risk of acute altitude sickness in humans. Background technique [0002] Acute mountain sickness (AMS) occurs in plain people who have lived in low-altitude areas for a long time, 1-3 days after entering the plateau quickly without adapting to the new environment, including headache, dizziness, fatigue, insomnia , Emotional restlessness, flatulence, diarrhea and vomiting and a series of symptoms, among which severe headache is a typical symptom of AMS. In geography, a vast area with an altitude higher t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
Inventor 黄河张二龙高钰琪徐刚何姝董华平柯贤峰陈建李鹏孙滨达
Owner ARMY MEDICAL UNIV
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