Immunofluorescence kit and application for detection of eggshell precursor protein of flounder flounder
A precursor protein and immunofluorescence technology, applied in the field of environmental detection, can solve the problems of lack of location and quantitative detection of eggshell precursor protein, obtain eggshell precursor protein, and not establish eggshell precursor protein, etc., to improve detection sensitivity The effect of high specificity and low background value
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[0023] An immunofluorescent kit for detecting eggshell precursor protein of brown flounder, characterized in that the kit is equipped with one FITC-labeled mouse polyclonal antibody against eggshell precursor protein of brown flounder.
[0024] The immunofluorescence detection technique for preparing and detecting the eggshell precursor protein of brown teeth comprises the following steps:
[0025] (1) Preparation of pure flounder eggshell precursor protein;
[0026] (2) Preparation of polyclonal antibody against eggshell precursor protein of flounder flounder;
[0027] (3) Preparation of FITC-labeled polyclonal antibody to eggshell precursor protein of brown flounder;
[0028] Wherein, the (1) method for preparing pure flounder eggshell precursor protein is as follows:
[0029] (1) Preparation of pure flounder eggshell precursor protein
[0030] ① Construction of prokaryotic expression vector for eggshell precursor protein
[0031]A large amount of Chg H mRNA was induced ...
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