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Primer and probe for synchronous detection of sheep-origin and pig-origin in meat product and detection method thereof

A meat product, sheep-derived technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of reducing mismatches

Pending Publication Date: 2019-06-14
XILINGOL VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, technical research, testing standards, invention patents and commercial kits related to authenticity identification of meat products are mainly focused on single-channel single-source detection and heterogeneous-tube quality control detection, while multi-channel multi-source detection and same-tube quality control detection Less reported

Method used

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  • Primer and probe for synchronous detection of sheep-origin and pig-origin in meat product and detection method thereof
  • Primer and probe for synchronous detection of sheep-origin and pig-origin in meat product and detection method thereof
  • Primer and probe for synchronous detection of sheep-origin and pig-origin in meat product and detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] 1. Detection method:

[0029] (1) Extract the DNA of fresh meat or processed meat products, and set up a blank control for extraction (the control group for subsequent extraction methods).

[0030] (2) Detect the concentration and quality of DNA, and dilute the concentration to about 100ng / μL.

[0031] (3) Use multiple fluorescent quantitative PCR primers and probes to amplify and detect diluted DNA, use sheep and pig positive standards as positive controls, use sterilized deionized water as negative controls, and use DNA extraction blank controls as extractions For the control group of the method, the Real-time PCR reaction system is shown in Table 1, and the Real-time PCR amplification parameters are shown in Table 4.

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Abstract

The invention discloses a primer and probe for synchronous detection of a sheep-origin and pig-origin in meat products and a detection method thereof. The primer and probe sequence are as follows: thesequence of a two-origin detection forward primer is shown as SEQ ID No. 1; the sequence of a sheep-origin detection reverse primer is shown as SEQ ID No. 2; the sequence of a pig-origin detection reverse primer is shown as SEQ ID No. 3; the sequence of a sheep probe is shown as SEQ ID No. 4; the sequence of a pig probe is shown as SEQ ID No. 5; and the sequence of a quality control probe is shown as SEQ ID No. 6. According to the primer and probe for synchronous detection of the sheep-origin and pig-origin in the meat products and the detection method thereof, the primer and probe have goodspecificity and high sensitivity, can realize sheep-origin, pig-origin and quality control same-tube detection in the meat products, and can carry out sheep-origin and pig-origin quantitative detection.

Description

technical field [0001] The invention belongs to the technical field of food detection, in particular to the field of detection of animal origin in meat products. Background technique [0002] Mutton is one of the livestock meats widely consumed by consumers. Mutton is rich in nutrition, tender and easy to digest, high in protein, low in fat, rich in phospholipids, and low in cholesterol. At the same time, there are no religious or cultural taboos for eating mutton. The 203,000-square-kilometer Xilin Gol League has 180,000 square kilometers of high-quality natural pastures. It is a traditional animal husbandry production area and has superior conditions for the development of modern animal husbandry. It is an important green animal product export base for the country and the Inner Mongolia Autonomous Region. In addition to being rich in conventional nutrients, meat products have a sensory experience of excellent color, aroma and taste, which endows them with the core of glob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11C12Q1/6851
Inventor 郭梁刘国强罗建兴海小郭元晟雅梅钱俊平
Owner XILINGOL VOCATIONAL COLLEGE
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