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Urine glucose test paper capable of resisting ascorbic acid interference and preparation method thereof

A technology for detecting test strips and ascorbic acid, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve problems such as ascorbic acid interference, and achieve accurate test results. The effect of anti-VC interference ability

Inactive Publication Date: 2019-06-07
DIRUI MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention will solve the technical problem of a large amount of ascorbic acid interference when the existing dry chemical test paper method detects urine glucose, and provides a urine glucose detection test paper interfered with by ascorbic acid and a preparation method thereof. The urine glucose detection test paper provided by the invention makes the ascorbic acid concentration low When it is higher than 3.5mmol / L, there is no interference in the urine glucose test, and there will be no false negative results in clinical practice

Method used

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  • Urine glucose test paper capable of resisting ascorbic acid interference and preparation method thereof
  • Urine glucose test paper capable of resisting ascorbic acid interference and preparation method thereof
  • Urine glucose test paper capable of resisting ascorbic acid interference and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048](1) Preparation of immersion solution 1: Prepare the immersion solution according to the following formula.

[0049]

[0050] (2) Preparation of immersion solution 2: Prepare the immersion solution according to the following formula.

[0051]

[0052]

[0053] The pH value of the above-mentioned citric acid / sodium citrate buffer solution is 6.5;

[0054] (3) Test paper preparation:

[0055] Ambient temperature requirement: 23-25℃, ambient humidity requirement: 20-30%;

[0056] The filter papers were completely immersed in immersion solutions 1 and 2, respectively, and dried at 5°C for 25 minutes. The dried filter papers were cut and fixed with the substrate, and rolled and cut to prepare urine glucose test papers 1-1 and 1-2.

[0057] (4) The prepared test papers 1-1 and 1-2 were used to measure the 5.6mmol / L glucose standard solution added with 3.5mmol / L ascorbic acid, and interpreted with a urine analyzer. The results are shown in the table below. (Neg mean...

Embodiment 2

[0060] (1) Preparation of immersion solution 1: Prepare the immersion solution according to the following formula.

[0061]

[0062]

[0063] (2) Preparation of immersion solution 2: Prepare the immersion solution according to the following formula.

[0064]

[0065] The pH value of the above-mentioned phosphate buffer solution is 5.5;

[0066] (3) Test paper preparation:

[0067] Ambient temperature requirement: 23-25℃, ambient humidity requirement: 20-30%;

[0068] The filter papers were completely immersed in immersion solutions 1 and 2, respectively, and dried at 60° C. for 20 minutes. After the dried filter papers were cut, they were combined with the substrate and fixed, and rolled and cut to prepare urine glucose test papers 2-1 and 2-2.

[0069] (4) The prepared test papers 2-1 and 2-2 were used to measure the glucose standard solution of 5.6mmol / L added with 3.5mmol / L ascorbic acid, and interpreted with a urine analyzer. The results are shown in the table b...

Embodiment 3

[0072] (1) Preparation of immersion solution 1: Prepare the immersion solution according to the following formula.

[0073]

[0074] (2) Preparation of immersion solution 2: Prepare the immersion solution according to the following formula.

[0075]

[0076] The pH value of the above-mentioned citric acid / sodium citrate buffer solution is 6.5;

[0077] (3) Test paper preparation:

[0078] Ambient temperature requirement: 23-25℃, ambient humidity requirement: 20-30%;

[0079] The filter papers were completely immersed in immersion solutions 1 and 2, respectively, and dried at 55° C. for 22 minutes. After the dried filter papers were cut, they were combined with the substrate and fixed, and rolled and cut to prepare urine glucose test papers 3-1 and 3-2.

[0080] (4) The prepared test papers 3-1 and 3-2 were used to measure the 5.6mmol / L glucose standard solution added with 3.5mmol / L ascorbic acid, and interpreted with a urine analyzer, the results are shown in the table...

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Abstract

The invention belongs to the field of urine analysis and detection, relates to urine glucose test paper capable of resisting ascorbic acid interference and a preparation method thereof and solves thetechnical problem of existence of ascorbic acid interference in the detection of urine glucose in an existing dry chemistry test paper. The urine glucose test paper capable of resisting ascorbic acidinterference is formed by a substrate and filter paper fixedly arranged on the substrate. The test paper is obtained by being immersed into immersion liquid consisting of a buffer solution, glucose oxidase, peroxidase, potassium iodide, ascorbic acid oxidase, anti-interference substance xanthan gum, a surfactant, an enzyme stabilizer and a blue dye substance, so that anti-VC interference capability of the glucose test paper with potassium iodide as the substrate can be significantly improved, and sensitivity and accuracy of the detection result are enhanced; and the glucose detection result isnot influenced when the content of the VC in the sample does not exceed 3.5mmol / L. The preparation method of the urine glucose test paper capable of resisting ascorbic acid interference is simple andeasy to operate, stable in performance and accurate in test result.

Description

technical field [0001] The invention belongs to the field of urine analysis and detection, and in particular relates to a urine glucose detection test paper interfered with by ascorbic acid and a preparation method thereof. Background technique [0002] Urinalysis is one of the routine tests in medical laboratories, plays an important role in medical tests, and has an important guiding role in the diagnosis and treatment of related diseases. Urinalysis can perform qualitative and quantitative analysis of various components in urine, including urobilinogen, bilirubin, ketone bodies, blood, protein, nitrite, white blood cells, glucose, creatinine, calcium, specific gravity, pH, Ascorbic acid and other items, among which the detection of urine glucose is the main detection item of urinalysis, and the results of urine glucose detection have important guiding significance for the evaluation of kidney-related diseases. [0003] There are three main methods for the detection of ur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 王丹凤魏学芹林月孙成艳何浩会
Owner DIRUI MEDICAL TECH CO LTD
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