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Ascorbic-acid-interference-resisting urine occult blood test paper and preparation method thereof

A technology for detecting ascorbic acid and test paper, which is applied in the direction of measuring devices, material analysis by observing the influence on chemical indicators, instruments, etc., can solve the problems of false negative of ascorbic acid samples, achieve stable performance, accurate test results, and adaptability wide range of effects

Pending Publication Date: 2020-12-01
JILIN GETEIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the existing detection method is prone to false negatives when testing samples containing ascorbic acid, and to provide a urine occult blood detection test paper interfered with by ascorbic acid and its preparation method

Method used

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  • Ascorbic-acid-interference-resisting urine occult blood test paper and preparation method thereof
  • Ascorbic-acid-interference-resisting urine occult blood test paper and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of infusion A: 0.03g of citric acid, 1.5g of sodium citrate, 50mL of purified water, 0.8g of sodium lauryl sulfate, and 0.9g of potassium iodate.

[0028] Preparation of infusion B: 200mL ethanol, 8g sodium lauryl sulfate, 1.5g 3,3',5,5'-tetramethylbenzidine, 1.0g di-tert-butyl cumene peroxide, 6-methyl Oxyquinoline 6mL, Hydrazine Yellow 170mg, Orange Yellow G 70mg.

[0029] After the preparation of liquid A and liquid B is completed, first fully immerse the filter paper in liquid A of the impregnating solution, take it out, and dry it at 70°C for 30 minutes, then immerse the dried filter paper in liquid B at 60°C and dry it for 10 minutes. The final filter paper is fixed to the base plate and cut into rectangular paper strips to obtain a urine occult blood test paper 1 interfered with by ascorbic acid.

[0030] With the prepared test paper 1, two groups of urine occult blood standard solutions with concentration gradients, wherein a group of urine occult b...

Embodiment 2

[0033] Preparation of infusion solution A: 0.1 g of citric acid, 1.3 g of sodium citrate, 50 mL of purified water, 0.6 g of sodium lauryl sulfate, and 0.9 g of potassium iodate.

[0034] Preparation of infusion solution B: 200 mL of ethanol, 12 g of sodium lauryl sulfate, 3.5 g of 3,3',5,5'-tetramethylbenzidine, 1.0 g of di-tert-butyl cumene peroxide, 6-methanol Oxyquinoline 6mL, Hydrazine Yellow 170mg, Orange Yellow G 70mg.

[0035] After the preparation of liquid A and liquid B is completed, first fully immerse the filter paper in liquid A of the impregnating solution, take it out, and dry it at 70°C for 30 minutes, then immerse the dried filter paper in liquid B at 60°C and dry it for 10 minutes. The final filter paper is fixed to the base plate and cut into rectangular paper strips to obtain a urine occult blood test paper 2 interfered with by ascorbic acid.

[0036] With the prepared test paper 2, two groups of urine occult blood standard solutions with concentration gra...

Embodiment 3

[0039] Preparation of infusion A: 0.3g of citric acid, 2g of sodium citrate, 50mL of purified water, 0.3g of sodium lauryl sulfate, and 1.3g of potassium iodate.

[0040]Preparation of infusion B: ethanol 100mL, sodium lauryl sulfate 3g, 3,3',5,5'-tetramethylbenzidine 0.5g, di-tert-butyl cumene peroxide 1.0g, 6-methanol Oxyquinoline 6mL, Hydrazine Yellow 170mg, Orange Yellow G 70mg.

[0041] After the preparation of liquid A and liquid B is completed, first fully immerse the filter paper in liquid A of the impregnating solution, take it out, and dry it at 70°C for 30 minutes, then immerse the dried filter paper in liquid B at 60°C and dry it for 10 minutes. The final filter paper is fixed to the base plate and cut into rectangular paper strips to obtain a urine occult blood test paper 3 interfered with by ascorbic acid.

[0042] With the prepared test paper 3, two groups of urine occult blood standard solutions with concentration gradients, one group of urine occult blood sta...

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Abstract

The invention relates to the field of urine analysis and detection, in particular to an ascorbic-acid-interference-resisting urine occult blood detection test paper and a preparation method thereof. The invention aims to solve the problem that false negative easily occurs when a sample containing ascorbic acid is tested by an existing detection method, and provides the ascorbic-acid-interference-resisting urine occult blood test paper and the preparation method thereof. The urine occult blood test paper comprises a support layer and a detection reagent block fixed on the support layer, whereinthe detection reagent block is obtained by soaking filter paper with a soaking solution A and carrying out, and then soaking the paper with a soaking solution B and carrying out drying. According tothe urine occult blood detection test paper provided by the invention, when the concentration of ascorbic acid is not higher than 5.0 mmol / L, the urine occult blood detection is not interfered, and afalse negative result does not appear clinically.

Description

technical field [0001] The invention relates to the field of urine analysis and detection, in particular to a urine occult blood detection test paper interfered with by ascorbic acid and a preparation method thereof. Background technique [0002] Urinary occult blood (urinary occult blood) is the presence of latent red blood cells in the urine that cannot be seen with the naked eye. There are no red blood cells or occasional traces of red blood cells in the urine of normal people, 0 to 2 per high-power lens field of view. After strenuous exercise, heavy physical labor or standing for a long time, there may be transient traces of red blood cells in the urine, which is normal. clinical significance. However, if there are more red blood cells in the urine, it indicates abnormality and should be checked closely. Most of the hematuria is caused by diseases of the urinary system itself. An increase in urinary red blood cells is a dangerous signal. Common causes of urinary red bl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759G01N2021/775
Inventor 刘潇璐徐李鹏刘玲
Owner JILIN GETEIN BIOTECH CO LTD
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