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Aphid natural enemy ladybug attractant

A natural enemy ladybug and attractant technology, applied in the direction of attracting pests, biocides, animal repellents, etc., can solve the problems of instability, poor effect, lack of applicability and specificity, and achieve stability High, good effect repeatability, good lure effect

Active Publication Date: 2021-06-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the invention patent "An Attractant for Seven-spot Ladybug" (CN201110180376.1), the raw materials of the attractant are composed of triple superphosphate, diammonium, and plant ash, which can attract adult seven-spot ladybugs, but the substances in this attractant have no The compound that specifically attracts ladybugs is a rough formula formed based on experience, which does not have applicability and specificity; patent CN201810000523.4 reports a red-ringed ladybug attractant for preventing and controlling paramecium, and the attractant is mainly paramecia The main volatile components produced by scales at different ages. This volatile is the kaimonin produced by pests. It can guide natural enemies to accurately locate pests at close range, but it involves long-distance luring. The effect of this formula is obviously poor. The number of ladybugs cannot be deployed in a large-scale overall area; CN201610297849.9 reports a kind of attractant for predatory natural enemy insects of aphids, which converts plant volatiles (mutual elements): α-pinene, eugenol, sophora The mixture of tree flower essential oil and chamomile essential oil has an attractive effect on ladybug heterochromia, ladybug six-spotted moon, hoverfly black belt, and lacewing; in CN201510579807.X, the pest-induced volatile matter (mutene): water Methyl sylate, 2-phenylethyl alcohol, phenylacetaldehyde, α-terpineol, etc. were mixed to prepare a natural enemy attractant for cotton aphids
Therefore, the effect of only using mutualene to lure natural enemies is unstable

Method used

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  • Aphid natural enemy ladybug attractant
  • Aphid natural enemy ladybug attractant
  • Aphid natural enemy ladybug attractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, aphid natural enemy ladybug attractant:

[0028] 0.01 mg of α-buchenene, 0.01 mg of α-humulene, 0.005 mg of E-β-farnesene, 0.07 mg of trans-2-hexenoic acid, 0.07 mg of benzaldehyde, 0.03 mg of isovaleraldehyde, and Dissolve 0.03 mg of alkenes, 0.12 mg of methyl salicylate, and 0.12 mg of eugenol in 20 μL of n-hexane, and mix well. The above solution was evenly added dropwise onto a quantitative filter paper sheet (5 cm in length and 1.5 cm in width) to obtain a compound loaded on a filter paper strip.

[0029] The behavioral response of ladybugs to this attractant was determined by wind tunnel experiments using a wind tunnel setup. The wind tunnel device is 90cm long, 35cm wide and 35cm high. 30 minutes before the start of the test, the required ladybugs were placed in a glass tube, and the two ends of the glass tube were sealed with absorbent cotton to adapt to the environment in the wind tunnel. The compound loaded on the filter paper is placed at the...

Embodiment 2

[0048] Attractant 1: Mix 0.01mg 1-vinyl-1-methyl-2,4-di(prop-1-en-2-yl)cyclohexane, 0.005mg E-β-farnesene, 0.07mg trans- 2-hexenoic acid, 0.03 mg isovaleraldehyde, and 0.12 mg phenylacetaldehyde were dissolved in 20 μL of n-hexane solution, mixed evenly, and prepared as attractant 1, and the above solution was evenly added dropwise on a quantitative filter paper sheet (length 5 cm, width 1.5 cm).

[0049] Attractant 2: Dissolve 0.01mg α-humulene, 0.005mg E-β-farnesene, 0.07mg 2,5-hexanedione, 0.03mg dipentene, 0.12mg leaf alcohol in 20μL n-hexane solution, Mix evenly to prepare attractant 2. Take 20 μL of the above solution and drop evenly onto a quantitative filter paper sheet (length 5 cm, width 1.5 cm).

[0050] Attractant 3: Dissolve 0.01 mg α-buchenene, 0.005 mg E-β-farnesene, 0.07 mg benzaldehyde, 0.03 mg isovaleraldehyde, and 0.12 mg squalene in 20 μL n-hexane solution, mix well, and prepare To form attractant 3, take 20 μL of the above solution and evenly drop it ont...

Embodiment 3

[0059] Dissolve 0.01mg of α-buchenene, 0.005mg of E-β-farnesene, 0.07mg of benzaldehyde, 0.03mg of isovaleraldehyde, and 0.12mg of squalene in 20μL of n-hexane, mix well, take the above solution and add it dropwise onto a quantitative filter paper sheet (length 5cm, width 1.5cm).

[0060] The behavioral response of ladybugs to this attractant was determined using a wind tunnel setup. The wind tunnel device is 90cm long, 35cm wide and 35cm high. 30 minutes before the start of the test, the required ladybugs were placed in a glass tube, and the two ends of the glass tube were sealed with absorbent cotton to adapt to the environment in the wind tunnel. The compound loaded on the filter paper is placed at the upper air outlet, and the wind speed in the wind tunnel is 0.3m / s. The whole bioassay process was carried out in a room at 28°C. Place the glass tube containing the ladybugs at the downwind end of the wind tunnel, and start timing for 30 minutes. Record the proportion of ...

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PUM

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Abstract

The invention discloses an attractant for ladybugs, a natural enemy of aphids, which comprises ladybug sex pheromone, mutene and kaiton; mutene is a volatile matter induced by aphids; and aphid honeydew volatiles. The ladybug attractant has good attracting effect, good effect repeatability and high stability no matter in laboratory or field test, and has broad-spectrum to beneficial ladybugs such as seven-spot ladybug, heterochromia ladybug and mole beetle. sex.

Description

technical field [0001] The invention relates to an attractant for pests, in particular to an attractant for ladybugs, a natural enemy of aphids. Background technique [0002] As one of the important means of comprehensive management of pests, biological control has the characteristics of high benefit, no pollution, safe for humans and animals, and conducive to environmental protection. With the development of modern agriculture, it has been widely valued. As a special resource, natural enemy insects It is also more and more accepted by people. Developed countries began to develop the natural enemy insect industry since the 1960s. At present, about 150 kinds of natural enemies are commercially produced and sold in the world, the main species are Trichogramma, grass ridge, predatory mite, small flower bug and ladybug, etc., and have achieved certain social and economic benefits. [0003] Ladybug is a general term for insects belonging to the family Coccinoptera. Ladybugs hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N27/00A01N37/06A01N35/04A01N35/02A01N37/40A01N31/06A01N31/02A01N25/18A01P19/00
CPCA01N27/00A01N31/16A01N35/02A01N35/04A01N37/40A01N49/00A01N31/02A01N31/04A01N37/02
Inventor 赵金浩李中珊汪彪王忠燕程敬丽杜永均
Owner ZHEJIANG UNIV
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