Detection method of cassia twig, peony and anemarrhena decoction

Abstract

The invention provides a detection method of cassia twig, peony and anemarrhena decoction, where detecting is achieved by means of high-performance liquid chromatography. The detection method includesthe steps of 1) preparing reference solutions, to be specific, collecting controls of gallic acid, mangiferin, paeoniflorin, prim-o-glucosylcimifugin, liquiritin, 5-O-methylvisammioside, cinnamic acid and ammonium glycyrrhetate, and adding methanol solution respectively to obtain the reference solutions; 2) preparing a test solution, to be specific, adding methanol to dissolve lyophilized powderof cassia twig, peony and anemarrhena decoction to obtain the test solution; 3) sucking the reference solutions and the test solution, and injecting them into a liquid chromatograph. The detection method is suitable for detecting gallic acid, mangiferin, paeoniflorin, prim-o-glucosylcimifugin, liquiritin, 5-O-methylvisammioside, cinnamic acid and ammonium glycyrrhetate, allows overall quality of the cassia twig, peony and anemarrhena decoction to be controlled, also helps provide methodological basis for the study on the material basis of the cassia twig, peony and anemarrhena decoction, and is worthy of popularization and application.

Description

technical field [0001] The invention specifically relates to a detection method of Guizhi Shaoyao Zhimu Decoction. Background technique [0002] Guizhi Shaoyao Zhimu Decoction comes from "Synopsis of the Golden Chamber · Stroke, Lijie, Syndrome, Syndrome and Treatment". It has the functions of expelling wind and dehumidification, warming meridians and dispelling cold, nourishing yin and clearing heat. Rheumatoid arthritis (RA) belongs to the category of arthralgia in traditional Chinese medicine. Modern clinical and pharmacological studies have found that Guizhi Shaoyao Zhimu Decoction has definite curative effect on RA. The pathogenesis of RA is not yet clear, but studies have shown that the level of free radicals in the serum and joint fluid of RA patients and model animals increases, and free radicals play an important role in the damage of articular cartilage in RA diseases. Guizhi Shaoyao Zhimu Decoction can Effectively scavenge free radicals. [0003] At present, th...

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Problems solved by technology

[0003] At present, the research on Guizhi Shaoyao Zhimu Decoction mainly focuses on its pharmacodynamics research, and there are few reports on its material basis research, such as Dong Yunzhuo. HPLC simultaneously determines the content of 7 active ingredients in Guizhi Shaoyao Zhimu Decoction, Only 7 of them can be analyzed, and cinnamic acid, which has obvious active ingredients, cannot be detected, which cannot effectively reflect the quality of Guizhi Shaoyao Zhimu Decoction

The detection methods disclosed at this stage cannot effectively control the quality of Guizhi Shaoyao Zhimu Decoction

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