Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composite micro-ecological preparation and preparation method thereof

A technology of composite microecology and preparations, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of single species of microecological preparations and poor pertinence, so as to improve production and breeding benefits, The effect of increasing the specification

Inactive Publication Date: 2019-05-31
ZHEJIANG INST OF FRESH WATER FISHERIES
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a compound microecological preparation and its preparation method and application for the problem that the probiotics have a single strain and are not highly targeted.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite micro-ecological preparation and preparation method thereof
  • Composite micro-ecological preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] The invention also discloses a preparation method of a composite microecological preparation for aquatic products, comprising the following steps:

[0042] Step 1, preparing Bacillus lateralis bacterium liquid:

[0043] Step 1.1, activating the Bacillus lateralis stored at 4°C, and culturing it with tryptone soybean medium in a shake flask at 30-35°C for 24 hours;

[0044] Among them, the formula of tryptone soybean medium is as follows (g / L): tryptone 17.0, soybean peptone 3.0, sodium chloride 5.0, dipotassium hydrogen phosphate 2.5, glucose 2.5, pH 7.1-7.5, autoclaved at 121°C for 15 minute;

[0045] Step 1.2. Carry out primary liquid expansion culture of the activated Bacillus lateralis, the inoculum amount is 10%-20%;

[0046] Step 1.3: Carry out secondary liquid expansion culture on the Bacillus lateralosporus after the first-level liquid expansion culture, the inoculum amount is 10%-20%; prepare the Bacillus lateralis bacterium liquid.

[0047] Step 2, preparin...

Embodiment 1

[0062] Step 1, preparing Bacillus lateralis bacterium liquid:

[0063] Step 1.1, activating the Bacillus lateralis stored at 4°C, and culturing it with tryptone soybean medium in a shake flask at 32°C for 24 hours;

[0064] Among them, the formula of tryptone soybean medium is as follows (g / L): tryptone 17.0, soybean peptone 3.0, sodium chloride 5.0, dipotassium hydrogen phosphate 2.5, glucose 2.5, pH 7.1-7.5, autoclaved at 121°C for 15 minute;

[0065] Step 1.2, carry out first-level liquid expansion culture to the activated Bacillus lateralis, the inoculum size is 15%;

[0066] Step 1.3: Carry out secondary liquid expansion culture on the Bacillus lateralosporus after the first-level liquid expansion culture, and the inoculum amount is 15%; prepare the Bacillus lateralis bacterium liquid.

[0067] Step 2, preparing bacillus licheniformis bacterium liquid:

[0068] Step 2.1, activating the Bacillus licheniformis stored at 4°C, and culturing it with tryptone soybean medium ...

Embodiment 2

[0080] Step 1, preparing Bacillus lateralis bacterium liquid:

[0081] Step 1.1, activating the Bacillus lateralis stored at 4°C, and culturing it with tryptone soybean medium in a shake flask at 30°C for 24 hours;

[0082] Among them, the formula of tryptone soybean medium is as follows (g / L): tryptone 17.0, soybean peptone 3.0, sodium chloride 5.0, dipotassium hydrogen phosphate 2.5, glucose 2.5, pH 7.1-7.5, autoclaved at 121°C for 15 minute;

[0083] Step 1.2, perform primary liquid expansion culture on the activated Bacillus lateralis, the inoculum size is 20%;

[0084] Step 1.3: Carry out secondary liquid expansion culture on the Bacillus lateralosporus after the first-level liquid expansion culture, and the inoculum amount is 20%; prepare the Bacillus lateralis bacterium liquid.

[0085] Step 2, preparing bacillus licheniformis bacterium liquid:

[0086] Step 2.1, activating the Bacillus licheniformis stored at 4°C, and culturing it with tryptone soybean medium in a s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composite micro-ecological preparation for aquatic products and a preparation method thereof. The composite micro-ecological preparation for aquatic products is prepared from, by weight percentage, 30-40% of a bacillus laterosporus solution, 30-40% of a lactobacillus casei solution and 20-30% of a bacillus licheniformis solution, wherein the total weight percentage is 100%. The composite micro-ecological preparation for aquatic products can rapidly reduce COD, nitrogen phosphorus, ammonia nitrogen and nitrite nitrogen of culturing water to achieve the purpose of degrading organic matter.

Description

technical field [0001] The invention belongs to the technical field of aquaculture microorganisms, and in particular relates to a composite microecological preparation for aquaculture and a preparation method thereof. Background technique [0002] my country is a big producer and exporter of aquatic products, and the variety and output of aquatic products are increasing year by year. However, with the rapid development of intensive farming in recent years, the residual bait and feces have seriously affected the water quality of the farming water. The accumulation of these organic pollutants gives a large number of harmful bacteria the opportunity to reproduce, making the water quality continue to deteriorate, seriously weakening the resistance of fish and shrimp, leading to large-scale outbreaks of diseases, and destroying the healthy development of the aquaculture industry. [0003] In recent years, microecological preparations have been widely used in aquaculture water qu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/10C12R1/245
Inventor 沈锦玉刘忆瀚尹文林蔺凌云潘晓艺姚嘉赟徐洋曹铮夏焱春
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com