A parasite staining solution that does not require fixation

Abstract

The invention discloses a parasite staining solution without fixation, which comprises the following components by weight: 5-8% eosin; 2-5% azure; 3-6% methylene blue; 10-50% methanol; Glycerin 0.01~0.02%; Sodium Chloride 5~6%; Tween-201~2%; Tocopherol 0.5~1%; Nano Pearl Powder 0.2~0.8%; Ethyl Acetate 0.6~0.9%; The present invention is applicable to the dyeing of parasites, various microorganisms, blood cell smears, bone marrow smears, exfoliated cell smears, chromosome banding, cell apoptosis detection, etc., and only uses one dye solution without fixing, fixing and staining Combining two into one, multiple inspections such as parasites, germs, nuclear heterogeneous cells, and cell classification and counting can be performed on one film at the same time. The method is simple and fast, with strong staining power, clear coloring, complete shape, and easy to identify and detect High rate.

Description

technical field [0001] The invention relates to the technical field of hygienic preparations, in particular to a parasite staining solution without fixation. Background technique [0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, which not only cause harm to the health of the staff, but also pollute the environment. [0003] The coloring of parasites is single, the coloring is not clear, the shape is incomplete, and it is easy to identify a single type of parasite, while other types of parasites are not easy to identify, which reduces the detection rate. Contents of the invention [0004] The invention provides a parasite staining solution without fixation. [0005] The scheme of the present invention is: ...

AI Technical Summary

Problems solved by technology

[0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, not only causing harm to the health of the staff, but also polluting the environment

[0003] Single coloring for parasites, unclear coloring, incomplete shape, and easy to identify a single type of parasite, while other types of parasites are not easy to identify, reducing the detection rate