An in vivo electrophysiology-guided single-cell labeling device

A labeling device, single cell technology, applied in medical science, diagnostic recording/measurement, stereotaxic surgical instruments, etc. Effect

Inactive Publication Date: 2020-09-25
SHANDONG NORMAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

One method is that the characteristic discharge activity of neurons in the animal behavior state can be used as a sign of the brain function state; the other method is that the active brain area can be located and analyzed by histochemical methods, but these two methods cannot Determining structure-function associations at the single-cell level
Therefore, it is difficult to clarify the composition of neurons in different brain functional active areas and their respective functional characteristics

Method used

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  • An in vivo electrophysiology-guided single-cell labeling device
  • An in vivo electrophysiology-guided single-cell labeling device
  • An in vivo electrophysiology-guided single-cell labeling device

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Embodiment Construction

[0032] It should be pointed out that the following detailed description is exemplary and is intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0033] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0034] Stereotaxic instrument (Stoeling, USA), also known as brain fixation device, uses a three-dimensional ...

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Abstract

The invention discloses a single-cell marking device under guidance of somatosensory electrophysiology. The device comprises a brain three-dimensional positioning instrument, an Ag / AgCl electrode, a glass micro-tube, a signal collecting electrode, a tracer electrode, a power source and a signal collecting system, wherein a fixing base is arranged at the free end of a three-dimensional micro-operation instrument of the brain three-dimensional positioning instrument, the glass micro-tube is vertically fixed to the fixing base, and the lower end of the glass micro-tube extends downwards out of the fixing base; the glass micro-tube comprises a capillary tube body and a tip end arranged at the end of the capillary tube, and a runner of the tip end is communicated with a runner of the capillarytube body; one end of the Ag / AgCl electrode is inserted into the runner of the capillary tube body of the glass micro-tube, and the other end of the Ag / AgCl electrode is connected with the signal collecting system through the signal collecting electrode; one end of the tracer electrode is connected with the Ag / AgCl electrode, and the other end of the tracer electrode is connected with the power source. Neuron cells can be directionally marked.

Description

technical field [0001] The invention belongs to the field of cell labeling, and in particular relates to a single cell labeling device guided by in vivo electrophysiology and a preparation method thereof. The single cell labeling device combines cell electrophysiology recording technology and tracer ion permeability cell labeling technology , which can realize the display of the correlation between the structure and function of a single neuron in the body under the condition of a complete neural network. Background technique [0002] The electrical activity in a certain area of ​​the brain is a sign of a specific function or related behavior of an animal, and it is the sum of the activities of different neural circuits in dynamically related brain areas. Neural circuits are composed of a variety of neurons, each of which has its own characteristics in terms of morphological structure, intrinsic properties, molecular recognition, pathway connections, and neural projections. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B90/00A61B90/10A61B5/0478A61B5/0476
Inventor 王敏李敏陈大典王雪楠孙爽贾青妹郭梦男孙海基何峰
Owner SHANDONG NORMAL UNIV
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