Application of rice glutaredoxin gene osgrxc2 in breeding

Abstract

The invention belongs to the technical field of plant genetic engineering and particularly discloses an application of Oryza sativa Grxs (glutaredoxins) gene OsGrxC2 to breeding. The nucleotide sequence of the Oryza sativa Grxs gene OsGrxC2 is shown as SEQ ID NO:1, the overall length of the sequence is 384 bp, and the gene encodes 127 amino acids; the gene OsGrxC2 and a protein encoded by the gene can be used for controlling the abortion degree of plant embryos and have a dosage effect, seeds of homozygous plants genetically modified with the gene OsGrxC2 can have character segregation, embryo-free or embryo-degenerated materials are produced, and the seeds cannot be reserved for planting; meanwhile, 1000-rice-seed weight and rice yield of the homozygous plants genetically modified with the gene OsGrxC2 are increased, and yield and quality of rice are improved; therefore, the gene OsGrxC2 can be used for assisting in breeding novel embryo-free or embryo-degenerated Oryza sativa strains or used for cultivating novel Oryza sativa varieties with high 1000-seed weight and high rice yield and has broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering. More specifically, rice glutaredoxin gene OsGrxC2 application in breeding. Background technique [0002] Glutaredoxins (Grxs) are a class of glutathione-dependent oxidoreductases, which were first discovered in 1976 by Holmgren et al. in Escherichia coli lacking the Trx gene, and are commonly found in bacteria, viruses, mammals and plants A small molecular protein with a molecular weight of about 10-15 kDa. Grxs function by regulating the redox of disulfide bonds, and usually contain a conserved dithiol cys-x-x-cys or monothiol cys-x-x-ser active site. Grxs in plants are named after the fourth residue of the GrxS or GrxC active site as S or C, and are divided into CC-type, CPYC-type and CGFS-type according to the characteristics of the conserved active site residues (Lemaire et al. , 2004; Rouhier et al., 2004). Some cDNAs encoding Grxs proteins have been isolated from vari...

AI Technical Summary

Problems solved by technology

The main reason is that the genetic background of the parents currently used in the three-line hybrid rice is relatively narrow, limited by the restorer gene, and the potential of heterosis is limited

The breeding procedures and production links of the three-line method are complicated, resulting in a long period of breeding new combinations, low efficiency, many promotion links, and high seed costs. The second-line hybrid rice also has problems such as difficult seed production, which affects the further development of hybrid rice. develop

The yield of conventional rice is stable and the quality is good. However, my country's plant variety protection system is not perfect, which also affects its further application.

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