Method for establishing tissue culture regeneration system of Lagerstroemia fauriei Koehne

A technology of crape myrtle and tissue culture, applied in the field of establishment of a tissue culture regeneration system of crape myrtle, can solve the problems of difficulty in inducing somatic embryos, long breeding cycle, etc., and achieves simple proliferation mode, genetic stability, and high callus induction rate. Effect

Inactive Publication Date: 2019-05-17
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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Problems solved by technology

[0004] Due to the shortcomings of traditional genetic breeding such as blindness and long breeding cycle, modern cell engineering breeding and molecular breeding overcome these shortcomings
In recent years, great progress has been made in tissue culture research of woody plants, such as poplar, plum blossom, eucalyptus, plane tree, etc., whose regeneration can be completed through somatic embryogenesis or organogenesis, but for woody plants, from nutrition Organ induction of somatic embryos is very difficult

Method used

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Embodiment Construction

[0023] The invention provides a method for tissue culture regeneration system of Lagerstroemia flockii, the specific steps are as follows:

[0024] 1) Obtaining sterile vaccines

[0025] Harvest the well-developed seeds on the healthy mother tree of Lagerstroemia fuschii, manually remove the seed wings and prevent damage to the seed coat, and carry out disinfection: Rinse with tap water for 30 minutes, then treat with 75% ethanol for 30 seconds; Sterilize with 1% sodium hypochlorite for 15 minutes, then rinse with sterile water for 5 times, and sow in MS basic medium after disinfection to obtain robust seedlings;

[0026] 2) Induction of callus

[0027] Cut the leaves that have been aseptically cultured for 20 days in step (1), cut the main veins of the vertical leaves 3 to 4 times, keep the leaf margins on one side connected, and inoculate the leaves on the callus induction medium 1 / 2MS+6-BA with the front facing up 1.0mg / L +2, 4-D 1.0 + sucrose 30g / L + agar 7g / L, dark ...

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Abstract

The invention relates to a method for establishing a tissue culture regeneration system of Lagerstroemia fauriei Koehne. According to the method, sterile seedlings are obtained by Lagerstroemia fauriei Koehne seed germination, then tender leaves are cut to serve as explants, the explants are subjected to dedifferentiation culture, callus with high redifferentiation capacity is obtained, then the callus is used for redifferentiation culture and follow-up rooting culture, and regenerated plants are obtained. By using the sterile seedlings as the explants, the problems of difficult dedifferentiation and high pollution rate due to high differentiation degree of the traditional explants are solved, and the operation is simple and feasible. The method specifically comprises the following steps:1, obtaining of the sterile seedlings; 2, induction of the callus; 3, subculture and proliferation of the callus; 4, redifferentiation of the callus; 5, rooting culture; 6, plant regeneration. The callus proliferation times of the Lagerstroemia fauriei Koehne reaches 2.1 times, the tissue culture regeneration rate reaches 95% or higher, and a firm experimental foundation is laid for establishmentof a genetic transformation system of Lagerstroemia indica.

Description

technical field [0001] The invention relates to a method for establishing a crape myrtle tissue culture regeneration system, and belongs to the technical field of woody plant tissue culture. Background technique [0002] Crape Myrtle ( Lagerstroemia indica ) belonging to the family Chrysanthemum ( Lythraceae ) Lagerstroemia genus, also known as "hundred-day red", "full house". Lagerstroemia is native to China and is one of the traditional famous flowers in my country. Domestic applications mainly include Lagerstroemia grandiflora ( L. speciosa ), Fujian Ziwei ( L.limii ), Nan Ziwei ( L. subcostata ), etc., there are only two species of Lagerstroemia indica and Lagerstroemia indica widely cultivated abroad. Lagerstroemia indica is usually planted as a deciduous shrub or tree. Because of its smooth trunk, beautiful tree posture and strong adaptability, it is often cultivated as a street tree and streetscape tree. In some cities, Lagerstroemia indica is used as a city...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈红吴寒陆小清李乃伟周艳威张凡李云龙王传永
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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