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Method for analyzing metabonomics by taking amino acid and acyl carnitine as target in blood

A technology of acylcarnitine and metabolomics, applied in the field of blood LC-MS/MS metabolomics research, can solve problems such as the destruction of balance mechanism

Active Publication Date: 2019-05-14
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology

Eating too much protein, beyond the body's ability to regulate, will destroy the balance mechanism

Method used

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  • Method for analyzing metabonomics by taking amino acid and acyl carnitine as target in blood
  • Method for analyzing metabonomics by taking amino acid and acyl carnitine as target in blood
  • Method for analyzing metabonomics by taking amino acid and acyl carnitine as target in blood

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Embodiment Construction

[0082] The present invention is further illustrated by examples below, and the examples are only limited to illustrate the present invention for easy understanding, rather than limiting the present invention.

[0083] (1) Collect a certain number of fresh blood samples, separate the above-mentioned blood samples to obtain serum or plasma samples, and divide the above-mentioned serum or plasma samples into sample groups and control groups according to the identification results of blood sample contributors;

[0084] With the consent of the blood donors, the blood of normal people and patients with clinically confirmed lung cancer was collected using inert separation gel coagulation tubes, with at least 20 cases in each group. After the fresh blood sample is collected, let the fresh blood sample stand still in the blood collection device for 2 hours, centrifuge it at 3000rpm for 10 minutes, take the supernatant as a serum or plasma sample, and store it at -80°C;

[0085] (2), ea...

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Abstract

The invention relates to a method for analyzing metabonomics by taking amino acid and acyl carnitine as a target in blood, comprising the following steps: (1) collecting a certain number of fresh blood samples to obtain serum or plasma samples, and dividing the serum or plasma samples into a sample group and a contrast group according to an identification result of a blood sample contributor; (2)performing targeted metabonomics pretreatment on each blood sample in the sample group and the contrast group; (3) sending each blood sample in the sample group and the contrast group to a high performance liquid chromatography tandem mass spectrometer, performing accurate quantification on target objects to obtain specific numerical value of each target object of each blood sample in the sample group and the contrast group; (4) comparing the target objects of the sample group and the contrast group, and selecting a target object with obvious difference; and (5) building a radar model, drawingthe target object with obvious difference to the radar model and finding out the relation between the target objects with obvious difference.

Description

technical field [0001] The invention relates to the field of blood LC-MS / MS metabolomics research, in particular to a metabolomics analysis method using amino acids and acylcarnitines as targeted targets. Background technique [0002] Small molecule metabolites in the human body are important living substances involved in human metabolism, including amino acids, organic acids, etc. The level of small molecule metabolites in the body reflects the environment of the cells and has an important impact on the life activities of the body. [0003] As a component of protein in the body, amino acids are not only the basic unit of protein construction, but also play important physiological functions in the body: (1) play a role in nitrogen balance. When the quality and quantity of protein in the daily diet are appropriate, the amount of nitrogen ingested The amount of nitrogen excreted by feces, urine and skin is equal, which is called the total nitrogen balance. It's actually a ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/34G01N30/86G01N30/88
Inventor 倪君君许丽
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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