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Method and device for evaluating constipation risk and constipation degree based on various intestinal bacteria contents

A technology for constipation and risk, which is applied in the field of medical biological detection, and can solve the problems that there is no precise evaluation method for constipation in molecular biology

Active Publication Date: 2019-05-14
SHANGHAI BIOTECAN PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Constipation diagnosis and grading standards are mainly based on the Rome III constipation diagnostic criteria, which mainly use inquiry and communication methods, imaging examinations, and there is no accurate constipation evaluation method at the molecular biology level

Method used

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  • Method and device for evaluating constipation risk and constipation degree based on various intestinal bacteria contents
  • Method and device for evaluating constipation risk and constipation degree based on various intestinal bacteria contents
  • Method and device for evaluating constipation risk and constipation degree based on various intestinal bacteria contents

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Experimental program
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Effect test

Embodiment 1

[0125] A method for QPCR detection of intestinal bacteria in healthy people and constipation patients to evaluate constipation is provided, and the specific determination method is as follows:

[0126] 1.1 Reagents or materials

[0127] 1) PBS buffer or saline.

[0128] 2) Bacterial Genome DNA Extraction Reagent (Tianlong, Ex-DNA Bacterial Genome Extraction Kit, model: ZTLYX).

[0129] 3) RNA-free water.

[0130] 4) 2 x Glodstar (Conway Century).

[0131] 5) SYBR Green Ⅰ (Tiangen Biology).

[0132] 6) The bacterial primers to be tested and the internal reference primers (Table 1).

[0133] Table 1

[0134]

[0135] 1.2 Provide stool samples

[0136] Collection of stool samples (45 healthy volunteers, 15 patients with constipation): collect about 300-500 mg of stool into a stool sample collection tube, add nucleic acid protection solution, invert several times to fully mix. Store the sample collection tube at -80°C until use.

[0137] 1.3 Extraction of bacterial geno...

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Abstract

The invention provides a method and device for evaluating constipation risk and constipation degree based on various intestinal bacteria contents, particularly provides a set of biomarkers comprisingtwo or more biomarkers selected from the group consisting of bacteroides sp., faecalibacterium prausnitzii, akkermansia muciniphila, enterobacter sp., clostridium hathewayi, or a combination thereof.According to the method and device, bacteria related to constipation in the intestinal tract are taken as targets, the relative content of bacteria is rapidly and efficiently detected by adopting a fluorescence PCR technology, the method has the advantages of objectivity, timeliness, rapidness, accuracy and the like in clinical diagnosis of constipation, which can assist clinicians to select and formulate the best treatment scheme according to specific needs, the treatment period of the patient is greatly shortened, and the intestinal tract health is recovered as soon as possible.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to a method and a device for evaluating the risk of constipation and the degree of constipation based on the content of various intestinal bacteria. Background technique [0002] Functional constipation (Functional constipation, referred to as constipation) is a common clinical gastrointestinal functional disease. The clinical symptoms are mainly manifested as decreased defecation frequency (<3 times / week) / hard stool and difficult defecation, among which the duration of chronic constipation is ≥6 months. In my country, the prevalence of chronic constipation in adults is about 4%-6%, while the prevalence of constipation in the elderly over 60 years old is as high as 22%. The constipation diagnosis and grading criteria are mainly based on the Rome III constipation diagnostic criteria, which mainly use inquiry and communication methods, imaging examinations, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/6851C12Q1/06C12R1/145C12R1/01
Inventor 甄林青王丽君许骋徐汪洋高军晖龚建兵
Owner SHANGHAI BIOTECAN PHARMA
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