Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Metabolome analysis method for dried blood spots and other trace biological samples

An analysis method and technology of biological samples, applied in the field of biological analysis, to achieve the effects of high method reproducibility, reduced metabolite loss, and wide range

Inactive Publication Date: 2019-05-10
FUDAN UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a high-throughput, high-sensitivity, full-coverage metabolome detection and analysis method suitable for DBS and other trace biological samples for the deficiencies of existing DBS pretreatment extraction schemes and analysis methods for molecular Epidemiological etiology screening and pathogenic mechanism research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Metabolome analysis method for dried blood spots and other trace biological samples
  • Metabolome analysis method for dried blood spots and other trace biological samples
  • Metabolome analysis method for dried blood spots and other trace biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Application of DBS Specimen Metabolome Analysis Technology in Air Pollution Epidemiological Research

[0042] 1. Sample Extraction

[0043] Use a 6mm hole punch to obtain two blood spots of the same area, place them in a 2mL centrifuge tube, and accurately add 120 μL of 80% (volume ratio) pre-cooled methanol water containing 2% formic acid (FA) (refrigerated at 4°C) 24h), vortex and oscillate to mix evenly for 10s, and sonicate in ice water, the sonication step is 1min sonication, 1min stop, a total of 10 cycles, and stand at room temperature for 30min. Centrifuge for 10min (4°C, 16000g), filter the supernatant with a 0.22μm PTFE filter membrane (Ф13mm, Jinteng), put it into a chromatographic sample bottle equipped with a liner tube, put it into a sample box and store it at -20°C The refrigerator is waiting for UHPLC-QTOF-MS detection. 5 μL of each sample was mixed as a quality control sample (quality control, QC), which can be used to monitor the stability...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of biological analysis, and specifically relates to a metabolome analysis method for dried blood spots and other trace biological samples. The method provided by the invention comprises the following steps: performing metabolite extraction on the samples of dried blood spots; performing global non-targeted metabolomics analysis on dried blood spots, wherein UHPLC-QTOF-MS is used for non-targeted metabolic phenotypic analysis of DBS samples; and analyzing the composition of metabolites of dried blood spots by using the RPLC and HILIC chromatographicseparation mode and electrospray ionization (ESI) positive and negative ion mass spectrometry detection mode. The method provided by the invention greatly reduces the loss of metabolites of the samples in the process of pretreatment and the influence of metal ions on the analyte, has the characteristics of high flux, high sensitivity and high coverage of metabolites, and can be applied to analysisand research such as molecular epidemiology screening and pathogenic mechanisms.

Description

technical field [0001] The invention belongs to the technical field of biological analysis, in particular to a metabolome analysis method for dried blood spots and other trace biological samples. This method is suitable for molecular epidemiological etiology screening and analysis of pathogenic mechanism. Background technique [0002] Dried Blood Spot (DBS) is a sample collection method in which whole blood samples are collected on cardboard, and after solvent extraction, the information of the components to be detected is detected to reflect the patient's diagnostic results. Compared with other blood sample collection methods, DBS has the advantages of less sample volume, simple collection, and convenient transportation. It is especially suitable for blood sample collection in remote and resource-poor areas. Therefore, it has been accepted by more and more people in recent years. At present, DBS has been widely used in many large-scale disease screenings in the world, suc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06G01N30/88
Inventor 唐惠儒芦勤玮
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com