Benign prostatic hyperplasia resisting urtica hyperborean Jacq extract, preparation method, and application of benign prostatic hyperplasia resisting urtica hyperborean Jacq extract
A technique for nettle extract and prostatic hyperplasia, which is applied in the field of medicine and achieves the effects of simple preparation process, high extraction rate and good therapeutic effect
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Embodiment 1
[0036] Embodiment 1 is used for anti-benign prostatic hyperplasia plateau nettle extract and the composition comparison of other nettles
[0037] Collect 11 kinds of Urtica medicinal materials respectively, accurately weigh 1g of dried medicinal herb powder of 11 kinds of Urtica medicinal plants, put them in a 100mL round bottom flask, add 25mL of methanol, shake well, put them in a 70℃ water bath for ultrasonic extraction for 30min, and statically Keep it cold, centrifuge the supernatant, pass through a 0.45 μm microporous membrane, and set aside.
[0038] At room temperature, weigh 9 kg of dried nettle powder from plateau nettle, wet the dry coarse powder of nettle from highland with 95% ethanol, pour it into the percolation barrel, add 95% ethanol until the liquid level is higher than the surface of the medicinal material, soak overnight, Gradient percolation with 95% ethanol, 50% ethanol, and water in an amount 8-10 times the weight of the material in sequence, collecting ...
Embodiment 2
[0043] Example 2 Investigation on the Activity of Different Extracted Parts of Plateau Urtica
[0044]The present invention starts from the perspectives of plateau nettle extract on related androgen and cell proliferation. The main androgen is DHT, and excessive levels of DHT in prostate tissue can lead to benign prostatic hyperplasia. Androgens can also indirectly regulate polypeptide growth factors and their receptors, thereby regulating the growth of prostate cells. Inhibition of high expression of apoptosis factors in prostate tissue can also cause prostate hyperplasia. Prostate index and prostate histopathological detection can intuitively express the degree of benign prostatic hyperplasia. Therefore, the present invention uses detection of serum DHT and EGF levels, expression of EGF and Bcl-2 mRNA in prostate tissue, prostate index and histopathological changes of prostate as indicators to investigate the anti-prostatic hyperplasia effect of different extraction parts ...
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