Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of novel vascular graft

A technology of blood vessels and bioreactors, applied in the field of preparation of new vascular grafts, can solve problems such as source and strength limitations, and unsatisfactory results

Inactive Publication Date: 2019-04-30
关茜茹
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autovascular is widely used clinically, but due to the limitation of source and intensity, satisfactory results cannot be achieved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take 0.1 g of commercially available collagen and soak it in 100 ml of 0.2 mol / L acetic acid solution. Stir well to fully dissolve, then place in the glass tube of a tumble mixer and roll the coating. Pour off excess collagen solution after forming a uniform collagen film. Pour 0.1 g of commercially available polyethylene glycol into 100 ml of sterilized physiological saline and stir evenly, take an appropriate amount of ethylene glycol liquid and drop it on the collagen film, and roll the coating with a drum agitator. Press 1.25×10 5 Add the recipient's own bone marrow-differentiated smooth muscle cells at a concentration of 1 / ml, plant continuously for 2 days, and then simultaneously press 1.25×10 5 Individual / ml concentration planted recipient's own bone marrow differentiated fibroblasts. Planted continuously for 3 days respectively. Next, the recipient's own bone marrow vascular endothelial progenitor cells and vascular endothelial growth factor were planted at ...

Embodiment 2

[0014] Take 0.2 g of commercially available collagen and soak it in 100 ml of 0.5 mol / L acetic acid solution. Stir well to fully dissolve, then place in the glass tube of a tumble mixer and roll the coating. Pour off excess collagen solution after forming a uniform collagen film. Pour 0.5 g of commercially available polyethylene glycol into 100 ml of sterilized physiological saline and stir evenly, take an appropriate amount of ethylene glycol liquid and drop it on the collagen film, and roll the coating with a drum agitator. Press 1.25×10 5 Add the recipient's own bone marrow-differentiated smooth muscle cells at a concentration of 1 / ml, plant continuously for 2 days, and then simultaneously press 1.25×10 5 Individual / ml concentration planted recipient's own bone marrow differentiated fibroblasts. Planted continuously for 3 days respectively. Next, the recipient's own bone marrow vascular endothelial progenitor cells and vascular endothelial growth factor were planted at ...

Embodiment 3

[0016] Take 1.0 g of commercially available collagen and soak it in 100 ml of 0.01 mol / L acetic acid solution. Stir well to fully dissolve, then place in the glass tube of a tumble mixer and roll the coating. Pour off excess collagen solution after forming a uniform collagen film. Pour 0.1 g of commercially available polyethylene glycol into 100 ml of sterilized physiological saline and stir evenly, take an appropriate amount of ethylene glycol liquid and drop it on the collagen film, and roll the coating with a drum agitator. Press 1.25×10 5 Add the recipient's own bone marrow-differentiated smooth muscle cells at a concentration of 1 / ml, plant continuously for 2 days, and then simultaneously press 1.25×10 5 Individual / ml concentration planted recipient's own bone marrow differentiated fibroblasts. Planted continuously for 3 days respectively. Next, the recipient's own bone marrow vascular endothelial progenitor cells and vascular endothelial growth factor were planted at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides preparation of a novel vascular graft, belongs to the field of medical biomaterials, and mainly solves the problems of limited autologous blood vessel sampling, complicated operation, high risk of allogeneic blood vessels and poor compliance force or calcification of macromolecular material implantation in vivo currently in clinic. According to the characteristics of different structures and different tissues and cells of natural blood vessels, three layers of receptor own tissue cells are planted, the bearing mechanical parameters and blood flow conditions of blood vessels in vivo are finally simulated, the product is prepared, and the purpose of autologous blood vessel repair is achieved. The material prepared by the method is prepared by culturing a collagen and receptor own tissue cells in a vascular bioreactor, is close to autologous blood vessels, has good biocompatibility, has growth potential, is not easy to infect, has strong mechanical properties, can bear the transmural pressure and the compliance force of blood flow to blood vessel walls, has good anti-thrombosis and anti-platelet adhesion effects, and is beneficial for vascular repair in vasculartransplantation.

Description

technical field [0001] The present invention relates to the preparation of a novel vascular graft. It has strong mechanical properties, can withstand the transmural pressure and the compliance force of blood flow to the vessel wall, and has good anti-thrombosis and anti-platelet adhesion effects. Good biocompatibility. Vascular grafts for cardiovascular and cerebrovascular diseases. It belongs to the field of medical biomaterials. Background technique [0002] Arterial ischemic disease represented by atherosclerotic heart disease is one of the main causes of human death. The main treatment is arterial grafting. In addition, congenital heart disease is a common congenital malformation that requires surgical correction, and some complicated congenital heart disease operations also require the application of vascular grafts. In the past, most of the vascular grafts we used were polymer artificial materials, such as polyester, polytetrafluoroethylene, etc., and each had its...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/50A61L27/40A61L27/10A61L27/34A61L27/38
CPCA61L27/507A61L27/10A61L27/34A61L27/3804A61L27/3826C08L89/00
Inventor 关茜茹
Owner 关茜茹
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com